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凝溶胶蛋白在 3T3-L1 细胞中的抗氧化和伤口愈合特性。

Antioxidant and Wound Healing Property of Gelsolin in 3T3-L1 Cells.

机构信息

IMTECH Centre for Animal Resources & Experimentation (iCARE), CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India.

Division of Protein Science & Engineering, CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India.

出版信息

Oxid Med Cell Longev. 2020 Feb 12;2020:4045365. doi: 10.1155/2020/4045365. eCollection 2020.

DOI:10.1155/2020/4045365
PMID:32104532
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7038438/
Abstract

Delineation of factors which affect wound healing would be of immense value to enable on-time or early healing and reduce comorbidities associated with infections or biochemical stress like diabetes. Plasma gelsolin has been identified earlier to significantly enable injury recovery compared to placebo. This study evaluates the role of rhuGSN for its antioxidant and wound healing properties in murine fibroblasts (3T3-L1 cell line). Total antioxidant capacity of rhuGSN increased in a concentration-dependent manner (0.75-200 g/mL). Cells pretreated with 0.375 and 0.75 g/mL rhuGSN for 24 h exhibited a significant increase in viability in a MTT assay. Preincubation of cells with rhuGSN for 24 h followed by oxidative stress induced by exposure to HO for 3 h showed cytoprotective effect. rhuGSN at 12.5 and 25 g/mL concentration showed an enhanced cell migration after 20 h of injury in a scratch wound healing assay. The proinflammatory cytokine IL-6 levels were elevated in the culture supernatant. These results establish an effective role of rhuGSN against oxidative stress induced by HO and in wound healing of 3T3-L1 fibroblast cells.

摘要

阐明影响伤口愈合的因素对于实现及时或早期愈合以及减少与感染或糖尿病等生物化学应激相关的并发症非常重要。血浆凝胶蛋白已被确定为能显著促进伤口恢复，优于安慰剂。本研究评估了 rhuGSN 的抗氧化和伤口愈合特性在鼠成纤维细胞（3T3-L1 细胞系）中的作用。rhuGSN 的总抗氧化能力呈浓度依赖性增加（0.75-200μg/mL）。用 0.375 和 0.75μg/mL rhuGSN 预处理细胞 24 小时后，在 MTT 测定中细胞活力显著增加。细胞用 rhuGSN 预孵育 24 小时，然后用 HO 暴露 3 小时诱导氧化应激，显示出细胞保护作用。在划痕伤口愈合试验中，在损伤后 20 小时，rhuGSN 在 12.5 和 25μg/mL 浓度下显示出增强的细胞迁移。培养上清液中的促炎细胞因子 IL-6 水平升高。这些结果确立了 rhuGSN 在对抗 HO 诱导的氧化应激和 3T3-L1 成纤维细胞伤口愈合中的有效作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c76/7038438/43bc3f094625/OMCL2020-4045365.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c76/7038438/6aa6f2731209/OMCL2020-4045365.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c76/7038438/80ffa74462a1/OMCL2020-4045365.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c76/7038438/4d9721c8fc90/OMCL2020-4045365.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c76/7038438/f06e68d9947d/OMCL2020-4045365.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c76/7038438/1d35e85860a6/OMCL2020-4045365.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c76/7038438/43bc3f094625/OMCL2020-4045365.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c76/7038438/6aa6f2731209/OMCL2020-4045365.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c76/7038438/80ffa74462a1/OMCL2020-4045365.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c76/7038438/4d9721c8fc90/OMCL2020-4045365.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c76/7038438/f06e68d9947d/OMCL2020-4045365.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c76/7038438/1d35e85860a6/OMCL2020-4045365.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c76/7038438/43bc3f094625/OMCL2020-4045365.006.jpg

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