Russo Daniela, Lela Ludovica, Benedetto Nadia, Faraone Immacolata, Paternoster Gianluca, Valentão Patricia, Milella Luigi, Carmosino Monica
Department of Health Science, University of Basilicata, Via dell'Ateneo Lucano 10, 85100 Potenza, Italy.
REQUIMTE/LAQV, Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, R. Jorge Viterbo Ferreira, n° 228, 4050-313 Porto, Portugal.
Int J Mol Sci. 2025 Mar 12;26(6):2562. doi: 10.3390/ijms26062562.
The therapeutic potential of natural products has led to the exploitation of phytocomplexes for treating various skin conditions, including wounds. DC. has traditionally been used for wound healing, burns, and other ailments. In this study, dried roots of were extracted using a hydroalcoholic solution, and the phytochemical composition was analyzed through HPLC-DAD. The polyphenol and polysaccharide content, along with in vitro antioxidant and anti-tyrosinase properties, were evaluated. The biological effect of extract was evaluated on the 3T3-L1 cell line. HPLC-DAD analysis confirmed the presence of several polyphenols, particularly caffeic acid derivatives, with echinacoside as the predominant compound, exhibiting strong antioxidant properties. The extract demonstrated no cytotoxic effect on 3T3-L1 cells, and it showed a protective effect by increasing the migration process in an in vitro scratch wound healing test, together with echinacoside and allantoin, which were used as references. Furthermore, the extract reduced the expression of proinflammatory cytokines and promoted that of proteins that accelerate wound closure, such as TGF1. The present study demonstrates the potential wound healing properties and the antioxidant and anti-inflammatory activity of root hydroalcoholic extract, giving a scientific rationale for its traditional use.
天然产物的治疗潜力促使人们开发植物复合物来治疗包括伤口在内的各种皮肤疾病。DC. 传统上一直用于伤口愈合、烧伤及其他疾病。在本研究中，使用水醇溶液提取了[植物名称]的干燥根，并通过HPLC-DAD分析其植物化学成分。评估了多酚和多糖含量以及体外抗氧化和抗酪氨酸酶特性。在3T3-L1细胞系上评估了[植物名称]提取物的生物学效应。HPLC-DAD分析证实存在几种多酚，特别是咖啡酸衍生物，其中紫锥菊苷为主要化合物，具有很强的抗氧化特性。该提取物对3T3-L1细胞无细胞毒性作用，并且在体外划痕伤口愈合试验中，与用作对照的紫锥菊苷和尿囊素一起，通过增加迁移过程显示出保护作用。此外，该提取物降低了促炎细胞因子的表达，并促进了加速伤口闭合的蛋白质（如TGF1）的表达。本研究证明了[植物名称]根水醇提取物具有潜在的伤口愈合特性以及抗氧化和抗炎活性，为其传统用途提供了科学依据。
