Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei, Taiwan (ROC).
Department of Orthopedic Surgery, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, Taiwan (ROC).
BMC Complement Med Ther. 2023 Jun 20;23(1):204. doi: 10.1186/s12906-023-04022-8.
Acemannan is an acetylated polysaccharide of Aloe vera extract with antimicrobial, antitumor, antiviral, and antioxidant activities. This study aims to optimize the synthesis of acemannan from methacrylate powder using a simple method and characterize it for potential use as a wound-healing agent.
Acemannan was purified from methacrylated acemannan and characterized using high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR), and H-nuclear magnetic resonance (NMR). 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assays were performed to investigate the antioxidant activity of acemannan and its effects on cell proliferation and oxidative stress damage, respectively. Further, a migration assay was conducted to determine the wound healing properties of acemannan.
We successfully optimized the synthesis of acemannan from methacrylate powder using a simple method. Our results demonstrated that methacrylated acemannan was identified as a polysaccharide with an acetylation degree similar to that in A. vera, with the FTIR revealing peaks at 1739.94 cm (C = O stretching vibration), 1370 cm (deformation of the H-C-OH bonds), and 1370 cm (C-O-C asymmetric stretching vibration); H NMR showed an acetylation degree of 1.202. The DPPH results showed the highest antioxidant activity of acemannan with a 45% radical clearance rate, compared to malvidin, CoQ10, and water. Moreover, 2000 µg/mL acemannan showed the most optimal concentration for inducing cell proliferation, while 5 µg/mL acemannan induced the highest cell migration after 3 h. In addition, MTT assay findings showed that after 24 h, acemannan treatment successfully recovered cell damage due to HO pre-treatment.
Our study provides a suitable technique for effective acemannan production and presents acemannan as a potential agent for use in accelerating wound healing through its antioxidant properties, as well as cell proliferation- and migration-inducing activities.
乙酰化甘露聚糖是从库拉索芦荟提取物中提取的乙酰化多糖，具有抗菌、抗肿瘤、抗病毒和抗氧化活性。本研究旨在采用简单的方法优化从甲基丙烯酰化粉末中合成乙酰化甘露聚糖，并对其进行表征，以作为一种潜在的伤口愈合剂。
从甲基丙烯酰化甘露聚糖中纯化得到乙酰化甘露聚糖，并采用高效液相色谱（HPLC）、傅里叶变换红外光谱（FTIR）和 H 核磁共振（NMR）进行表征。采用 2,2-二苯基-1-苦基肼（DPPH）和 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基-2H-四唑溴盐（MTT）法分别研究乙酰化甘露聚糖的抗氧化活性及其对细胞增殖和氧化应激损伤的影响。进一步进行迁移实验以确定乙酰化甘露聚糖的伤口愈合特性。
我们成功地采用简单的方法优化了从甲基丙烯酰化粉末中合成乙酰化甘露聚糖的方法。结果表明，甲基丙烯酰化甘露聚糖被鉴定为一种多糖，其乙酰化程度与库拉索芦荟中的乙酰化程度相似，FTIR 在 1739.94cm（C=O 伸缩振动）、1370cm（H-C-OH 键变形）和 1370cm（C-O-C 不对称伸缩振动）处有峰；H NMR 显示乙酰化程度为 1.202。DPPH 结果表明，乙酰化甘露聚糖的自由基清除率最高，达到 45%，与花青素、CoQ10 和水相比具有更高的抗氧化活性。此外，2000µg/mL 的乙酰化甘露聚糖表现出最佳的诱导细胞增殖浓度，而 5µg/mL 的乙酰化甘露聚糖在 3 小时后诱导细胞迁移的效果最佳。此外，MTT 实验结果表明，经过 24 小时处理后，乙酰化甘露聚糖成功恢复了 HO 预处理引起的细胞损伤。
本研究为有效生产乙酰化甘露聚糖提供了一种合适的技术，并表明乙酰化甘露聚糖作为一种潜在的伤口愈合剂，通过其抗氧化特性以及诱导细胞增殖和迁移的活性，可加速伤口愈合。
