Nanjing Maternity and Child Health Care Hospital, the Women's Hospital of Nanjing Medical University, Nanjing, Jiangsu 210004, China.
Department of Pediatrics, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453000, China.
Food Funct. 2020 Mar 26;11(3):2291-2298. doi: 10.1039/c9fo02813g.
A large number of bioactive peptides derived from breast milk have been identified to be multifunctional having anti-inflammatory, immunoregulatory and antimicrobial activities. Here, we report that an endogenous peptide located at β-casein 211-225 amino acid from human breast milk (hereafter called CAMP211-225) presents specific antimicrobial activity against pathogenic E. coli and Y. enterocolitica. CAMP211-225 is a novel peptide that occurs at higher levels in preterm milk than in term milk. The minimal inhibitory concentrations (MIC) of CAMP211-225 against E. coli and Y. enterocolitica are 3.125 μg ml-1 and 6.25 μg ml-1, respectively, and the antimicrobial activity of CAMP211-225 was also confirmed by a disk diffusion assay. Further studies using fluorescence staining, scanning electron microscopy and a DNA-binding assay revealed that CAMP211-225 kills bacteria through a membrane-disrupting mechanism, but not by binding to intracellular nucleic acids. Neonatal necrotizing enterocolitis (NEC) is a devastating gastrointestinal disease in neonatal intensive care units. In our study, CAMP211-225 administration effectively reduced ileal mucosa damage in an experimental NEC mice model. These results suggest that the antimicrobial peptide CAMP211-225 may have potential value in the prevention and treatment of neonatal infections.
大量源自母乳的生物活性肽已被鉴定为具有多功能性，具有抗炎、免疫调节和抗菌活性。在这里，我们报告说，一种源自人乳β-酪蛋白 211-225 个氨基酸的内源性肽（以下称为 CAMP211-225）对致病性大肠杆菌和肠耶尔森氏菌具有特定的抗菌活性。CAMP211-225 是一种新型肽，在早产儿乳中比在足月儿乳中含量更高。CAMP211-225 对大肠杆菌和肠耶尔森氏菌的最小抑菌浓度（MIC）分别为 3.125 μg ml-1 和 6.25 μg ml-1，并且 CAMP211-225 的抗菌活性也通过圆盘扩散试验得到了证实。进一步的荧光染色、扫描电子显微镜和 DNA 结合试验研究表明，CAMP211-225 通过破坏细胞膜的机制杀死细菌，而不是通过与细胞内核酸结合。新生儿坏死性小肠结肠炎（NEC）是新生儿重症监护病房中一种严重的胃肠道疾病。在我们的研究中，CAMP211-225 的给药有效减轻了实验性 NEC 小鼠模型中的回肠黏膜损伤。这些结果表明，抗菌肽 CAMP211-225 在预防和治疗新生儿感染方面可能具有潜在价值。
