Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte Sant'Angelo, Via Cintia 4, 80126 Naples, Italy.
Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Naples, Italy.
Pathog Dis. 2020 Apr 1;78(3). doi: 10.1093/femspd/ftaa012.
Staphylococcus epidermidis is well known to be one of the major causes of infections related to medical devices, mostly due to its strong capacity to form device-associated biofilms. Nowadays, these infections represent a severe burden to the public health system and the necessity of novel antibacterial strategies for the treatment of these difficult-to-eradicate infections is urgent. The Antarctic marine bacterium Pseudoalteromonas haloplanktis TAC125 was found to be able to produce an anti-biofilm molecule, the pentadecanal, active against S. epidermidis. In this work, we modified one of the most widely used silicone-based polymers, polydimethylsiloxane (PDMS), by adsorption of pentadecanal and its most promising derivative, pentadecanoic acid, on the PDMS surface. The biofilm formation of S. epidermidis RP62A on both untreated and modified PDMS was performed in a parallel plate flow chamber system, demonstrating the capability of the proposed anti-biofilm coatings to strongly reduce the biofilm formation. Furthermore, drug-release capacity and long-term efficacy (21 days) were also proven for the pentadecanoic acid coating.
表皮葡萄球菌是众所周知的医疗器械相关感染的主要原因之一，主要是由于其形成器械相关生物膜的强大能力。如今，这些感染对公共卫生系统造成了严重负担，迫切需要新的抗菌策略来治疗这些难以根除的感染。研究发现，南极海洋细菌假交替单胞菌 TAC125 能够产生一种抗生物膜分子十五烷醛，对表皮葡萄球菌具有活性。在这项工作中，我们通过在 PDMS 表面吸附十五烷醛及其最有前途的衍生物十五烷酸，对最广泛使用的硅基聚合物之一聚二甲基硅氧烷 (PDMS) 进行了修饰。在平行板流动腔系统中，对未经处理和改性的 PDMS 上的表皮葡萄球菌 RP62A 生物膜形成进行了研究，结果表明所提出的抗生物膜涂层能够强烈抑制生物膜形成。此外，还证明了十五烷酸涂层具有药物释放能力和长期疗效（21 天）。
