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从……获得的纯化合物对……的抗菌活性评估:初步结果。 (你提供的原文中“from”和“against”后缺少具体内容)

Antimicrobial activity evaluation of pure compounds obtained from against : Preliminary results.

作者信息

Venuti Iolanda, Ceruso Marina, D'Angelo Caterina, Casillo Angela, Pepe Tiziana

机构信息

Department of Veterinary Medicine and Animal Production.

Department of Agricultural Sciences.

出版信息

Ital J Food Saf. 2022 Jun 27;11(2):10320. doi: 10.4081/ijfs.2022.10320. eCollection 2022 Jun 21.

Abstract

is a foodborne pathogen responsible for a serious disease with a high mortality rate, particularly in vulnerable consumers. Recently, the scientific community has shown increasing attention to the search for new natural molecules with antimicrobial activity, aimed at preventing the spread of foodborne diseases. Extremophilic microorganisms, typical of extreme temperature environments, are a valuable source of these molecules. The present work aimed to study the antibacterial activity of four pure compounds derived from a molecule, the pentadecanal, produced by the Antarctic bacterium , against two different pathotypes of . Growth assays were performed in 96-well polystyrene plates with serial dilutions of the tested compounds at different concentrations (0.6, 0.3, 0.15, 0.07 mg/mL). The plates were incubated at 37°C for 24 h, with a spectrophotometric reading at OD 600 nm. Preliminary results of this study showed that pentadecanal inhibits the growth of , with a MIC (Minimum Inhibitory Concentration) of 0.6 mg/mL. Acetal, carboxylic acid, and ester did not demonstrate antibacterial activity at the concentrations tested. These findings suggest the possibility of using pentadecanal as a natural antibacterial to improve safety standards along the food supply chain.

摘要

是一种食源性病原体,会引发一种死亡率很高的严重疾病,在易感染消费者中尤为如此。最近,科学界越来越关注寻找具有抗菌活性的新天然分子,旨在防止食源性疾病的传播。极端嗜热微生物是极端温度环境中的典型微生物,是这些分子的宝贵来源。本研究旨在研究从南极细菌产生的一种分子十五醛衍生的四种纯化合物对两种不同致病型的抗菌活性。在96孔聚苯乙烯板中进行生长试验,将测试化合物以不同浓度(0.6、0.3、0.15、0.07mg/mL)进行系列稀释。将平板在37°C孵育24小时,在OD 600nm处进行分光光度读数。本研究的初步结果表明,十五醛可抑制的生长,最低抑菌浓度(MIC)为0.6mg/mL。在所测试的浓度下,缩醛、羧酸和酯未表现出抗菌活性。这些发现表明,有可能将十五醛用作天然抗菌剂,以提高食品供应链中的安全标准。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3be5/9272078/934e60b7dca4/ijfs-11-2-10320-g001.jpg

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