Department of Urology, China-Japan Union Hospital, Jilin University, Jilin, China.
Department of Endoscopy Center, China-Japan Union Hospital, Jilin University, Jilin, China.
Technol Cancer Res Treat. 2020 Jan-Dec;19:1533033819894573. doi: 10.1177/1533033819894573.
We aimed to explore the diagnostic value of microRNA-192 expression in urinary sediment combined with B-ultrasound in the diagnosis of bladder cancer.
A total of 118 patients with bladder cancer and 120 patients with benign urinary system diseases were selected for collection of urinary sediment. Real-time quantitative polymerase chain reaction was applied to detect the microRNA-192 expression (normalized to U6 level) in urinary sediment. Besides, the relationship between microRNA-192 expression and clinicopathological characteristics was analyzed. Furthermore, receiver operating characteristic curve was performed to analyze clinical value of microRNA-192 expression alone and microRNA-192 expression in urinary sediment combined with B-ultrasound in the diagnosis of bladder cancer.
MicroRNA-192 expression was significantly downregulated in urinary sediment of patients with bladder cancer, which was related to tumor stage and tumor size ( < .05). The results of receiver operating characteristic curve analysis showed that the best critical value of microRNA-192 expression in urinary sediment for the diagnosis of bladder cancer was 0.785 with the sensitivity and specificity of 76.7% and 78.0%, respectively. The sensitivity and specificity of microRNA-192 expression in urinary sediment combined with B-ultrasound in the diagnosis of bladder cancer were 93.2% and 76.7%, respectively. The sensitivity of combined diagnosis (93.2%) was not significantly different from that of cystoscopy (93.2%; > 0.05). There were significant differences between the expression of microRNA-192 in urinary sediment and the sensitivity of B-ultrasound examination alone with cystoscopy ( < .05).
The downregulation of microRNA-192 expression in urinary sediment of patients with bladder cancer may be related to tumor progression. The microRNA-192 expression in urinary sediment is valuable in the diagnosis of bladder cancer, which shows high sensitivity in diagnosis of bladder cancer when combined with B-ultrasound.
探讨尿沉渣中 microRNA-192 表达联合 B 超对膀胱癌的诊断价值。
选取膀胱癌患者 118 例和良性泌尿系统疾病患者 120 例，收集尿沉渣，采用实时荧光定量聚合酶链反应检测尿沉渣中 microRNA-192 的表达（以 U6 为内参），分析 microRNA-192 表达与临床病理特征的关系，绘制受试者工作特征曲线分析尿沉渣中 microRNA-192 表达单独及与 B 超联合对膀胱癌的诊断价值。
膀胱癌患者尿沉渣中 microRNA-192 表达明显下调，与肿瘤分期、肿瘤大小有关（<0.05）。受试者工作特征曲线分析结果显示，尿沉渣中 microRNA-192 表达诊断膀胱癌的最佳临界值为 0.785，敏感度和特异度分别为 76.7%和 78.0%。尿沉渣中 microRNA-192 表达联合 B 超诊断膀胱癌的敏感度和特异度分别为 93.2%和 76.7%。联合诊断的敏感度（93.2%）与膀胱镜检查（93.2%）差异无统计学意义（>0.05）。尿沉渣中 microRNA-192 表达与单独 B 超检查及膀胱镜检查敏感度差异均有统计学意义（<0.05）。
膀胱癌患者尿沉渣中 microRNA-192 表达下调可能与肿瘤进展有关。尿沉渣中 microRNA-192 表达对膀胱癌有诊断价值，与 B 超联合诊断膀胱癌时具有较高的敏感度。
