通过与“特权”tRNA 合成酶结合，用一种光活性氨基酸工程化溴结构域。
Engineering bromodomains with a photoactive amino acid by engaging 'Privileged' tRNA synthetases.
机构信息
Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA.
出版信息
Chem Commun (Camb). 2020 Mar 28;56(25):3641-3644. doi: 10.1039/c9cc09891g. Epub 2020 Feb 28.
Abstract
Site-specific placement of unnatural amino acids, particularly those responsive to light, offers an elegant approach to control protein function and capture their fleeting 'interactome'. Herein, we have resurrected 4-(trifluoromethyldiazirinyl)-phenylalanine, an underutilized photo-crosslinker, by introducing several key features including easy synthetic access, site-specific incorporation by 'privileged' synthetases and superior crosslinking efficiency, to develop photo-crosslinkable bromodomains suitable for 'interactome' profiling.
摘要
定点引入非天然氨基酸，特别是对光响应的非天然氨基酸，为控制蛋白质功能并捕获其短暂的“互作组”提供了一种优雅的方法。在此，我们通过引入几个关键特性，包括简便的合成方法、通过“特权”合成酶进行定点掺入以及更高的交联效率，复活了 4-(三氟甲基重氮基)苯丙氨酸这种未充分利用的光交联剂，开发了适用于“互作组”分析的光交联溴结构域。
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