自噬生物标志物在烧伤年龄估计及病情进展中的作用
Role of Autophagy Biomarkers In Burn-Age Estimation and Progression.
作者信息
Yassa Heba A, Hanna Randa T, El Abdin Hala Zein
出版信息
Acad Forensic Pathol. 2019 Sep;9(3-4):163-171. doi: 10.1177/1925362119891705. Epub 2020 Jan 31.
BACKGROUND
Determination of time passed since burn injury in the living is critical in forensic science. Autophagy biomarkers and vitronectin can play an important role in determination of the age of burn injuries through their levels in the tissue.
OBJECTIVE
The aim of this study was to investigate the role of autophagy biomarkers in dating burn injury and to correlate them with the histopathological effects of deep second-degree thermal burn.
METHOD
Fifty-four male rats were used in this study after infliction of second-degree thermal burns to their skin. Samples were taken from them after 30 minutes and one, four, 24, 48, and 72 hours following burn to be examined histologically and also for autophagy biomarkers and vitronectin.
RESULTS
Significant reduction in the autophagy biomarkers (p < 0.001) over the first 24 hours then began to increase but still not reach the normal level up to 72 hours after burn. Vitronectin level increased after burn infliction 1.5-fold after first hour, then up to four-fold after four hours and after that began to decline but still did not reach the normal level up to 72 hours.
CONCLUSION
Autophagy biomarkers can be used as a forensic tool in determination of the time passed since burn infliction in living.
背景
确定活体烧伤后的时间对于法医学至关重要。自噬生物标志物和玻连蛋白可通过其在组织中的水平在烧伤年龄的确定中发挥重要作用。
目的
本研究旨在探讨自噬生物标志物在烧伤时间鉴定中的作用，并将其与深二度热烧伤的组织病理学效应相关联。
方法
本研究使用54只雄性大鼠，对其皮肤造成二度热烧伤。在烧伤后30分钟以及1、4、24、48和72小时从大鼠身上取样，进行组织学检查以及自噬生物标志物和玻连蛋白检测。
结果
在烧伤后的前24小时内，自噬生物标志物显著降低（p < 0.001），随后开始升高，但直至烧伤后72小时仍未达到正常水平。玻连蛋白水平在烧伤后1小时增加1.5倍，4小时后增加至4倍，之后开始下降，但直至72小时仍未达到正常水平。
结论
自噬生物标志物可作为一种法医工具用于确定活体烧伤后的时间。
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