水鳖科（Hydrocharitaceae）某个变种的EST-SSR标记的开发与特性分析
Development and characterization of EST-SSR markers for var. (Hydrocharitaceae).
作者信息
Li Zhi-Zhong, Lu Meng-Xue, Saina Josphat K, Gichira Andrew W, Wang Qing-Feng, Chen Jin-Ming
机构信息
Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, People's Republic of China.
University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
出版信息
Appl Plant Sci. 2017 Nov 14;5(11). doi: 10.3732/apps.1700083. eCollection 2017 Nov.
PREMISE OF THE STUDY
Simple sequence repeat (SSR) markers were derived from transcriptomic data for (Hydrocharitaceae), a species comprising five endemic and highly endangered varieties in China.
METHODS AND RESULTS
Sixteen novel SSR markers were developed for var. . One to eight alleles per locus were found, with a mean of 2.896. The observed and expected heterozygosity ranged from 0.000 to 1.000 and 0.000 to 0.793, respectively. Interestingly, in cross-varietal amplification, 13 out of the 16 loci were successfully amplified in var. , and 12 amplified in each of the other three varieties of .
CONCLUSIONS
These newly developed SSR markers will facilitate further study of genetic variation and provide important genetic data needed for appropriate conservation of natural populations of all varieties of .
研究前提
简单序列重复（SSR）标记是从水鳖科植物（Hydrocharitaceae）的转录组数据中获得的，该物种在中国包含五个特有且高度濒危的变种。
方法与结果
为水鳖变种（Hydrocharis dubia var. japonica）开发了16个新的SSR标记。每个位点发现1至8个等位基因，平均为2.896个。观察到的杂合度和预期杂合度分别在0.000至1.000和0.000至0.793之间。有趣的是，在跨变种扩增中，16个位点中的13个在水鳖变种（Hydrocharis dubia var. tangutorum）中成功扩增，另外三个水鳖变种中的每一个都有12个位点扩增成功。
结论
这些新开发的SSR标记将有助于进一步研究遗传变异，并为妥善保护水鳖所有变种的自然种群提供所需的重要遗传数据。
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