丁香对产超广谱β-内酰胺酶（ESBL）、金属β-内酰胺酶（MBL）和AmpCβ-内酰胺酶的尿路病原体的抗菌活性：我们离获得一种新型抗菌剂还有多远？
Antibacterial activity of (clove) against uropathogens producing ESBL, MBL, and AmpC beta-lactamase: Are we close to getting a new antibacterial agent?
作者信息
Faujdar Sameer S, Bisht Dakshina, Sharma Amisha
机构信息
Department of Microbiology, Santosh Medical College and Hospital, Ghaziabad, Uttar Pradesh, India.
Department of Microbiology, Maharishi Markandeshwar Medical College and Hospital, Kumarhatti, Solan, Himachal Pradesh, India.
出版信息
J Family Med Prim Care. 2020 Jan 28;9(1):180-186. doi: 10.4103/jfmpc.jfmpc_908_19. eCollection 2020 Jan.
INTRODUCTION
The present study was done to access the antibacterial activity of clove () against extended-spectrum beta-lactamase (ESBL), metallo-beta-lactamase (MBL), and AmpC beta-lactamase-producing gram-negative bacteria causing urinary tract infection.
METHODS
A total of 221 gram-negative uropathogens were isolated and screened for beta-lactamase (ESBL, MBL, and AmpC) production and further tested against ethanolic extract of clove () for its antibacterial activity.
RESULTS
Clove was effective against all gram-negative isolates but the best antibacterial activity was shown against species with 19 mm zone of inhibition, 0.39 mg/ml minimum inhibitory concentration (MIC) and 0.19 mg/ml minimum bactericidal concentration (MBC).
CONCLUSIONS
Clove extract showed different antibacterial potential against all gram-negative uropathogens. Clove activity for particular strain was found to be similar between isolates producing beta-lactamase and non beta-lactamase.
引言
本研究旨在评估丁香对产超广谱β-内酰胺酶（ESBL）、金属β-内酰胺酶（MBL）和AmpCβ-内酰胺酶的革兰氏阴性菌引起的尿路感染的抗菌活性。
方法
共分离出221株革兰氏阴性尿路致病菌，筛选其β-内酰胺酶（ESBL、MBL和AmpC）的产生情况，并进一步检测丁香乙醇提取物的抗菌活性。
结果
丁香对所有革兰氏阴性分离株均有效，但对某菌种显示出最佳抗菌活性，抑菌圈直径为19毫米，最低抑菌浓度（MIC）为0.39毫克/毫升，最低杀菌浓度（MBC）为0.19毫克/毫升。
结论
丁香提取物对所有革兰氏阴性尿路致病菌显示出不同的抗菌潜力。发现丁香对产β-内酰胺酶和不产β-内酰胺酶的分离株中特定菌株的活性相似。
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