Laboratory for Stem Cell Research, Department of Health Science and Technology, Aalborg University, 9220 Aalborg, Denmark.
Pediatric Urology Section, Sidra Medicine, Doha 26999, Qatar.
Cells. 2020 Feb 26;9(3):538. doi: 10.3390/cells9030538.
In order to reconstruct injured urinary tract tissues, biodegradable scaffolds with autologous seeded cells are explored in this work. However, when cells are obtained via biopsy from individuals who have damaged organs due to infection, congenital disorders, or cancer, this can result in unhealthy engineered cells and donor site morbidity. Thus, neo-organ construction through an alternative cell source might be useful. Significant advancements in the isolation and utilization of urine-derived stem cells have provided opportunities for this less invasive, limitless, and versatile source of cells to be employed in urologic tissue-engineered replacement. These cells have a high potential to differentiate into urothelial and smooth muscle cells. However, urinary tract reconstruction via tissue engineering is peculiar as it takes place in a milieu of urine that imposes certain risks on the implanted cells and scaffolds as a result of the highly cytotoxic nature of urine and its detrimental effect on both growth and differentiation of these cells. Both of these projections should be tackled thoughtfully when designing a suitable approach for repairing urinary tract defects and applying the needful precautions is vital.
为了重建受损的尿路组织，本工作探索了具有自体种子细胞的可生物降解支架。然而，当通过活检从因感染、先天畸形或癌症而导致器官受损的个体中获得细胞时，这可能导致工程细胞不健康和供体部位发病率增加。因此，通过替代细胞来源进行新器官构建可能是有用的。从尿液中分离和利用干细胞的重大进展为这种微创、无限和多功能的细胞来源在泌尿科组织工程替代物中的应用提供了机会。这些细胞具有分化为尿路上皮和平滑肌细胞的高潜力。然而，通过组织工程进行尿路重建是特殊的，因为它发生在尿液环境中，由于尿液的高度细胞毒性及其对这些细胞的生长和分化的有害影响，对植入的细胞和支架带来了一定的风险。在设计修复尿路缺陷的合适方法时，应仔细考虑这两个方面，并且应用必要的预防措施至关重要。
