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三聚体 G 蛋白参与调控水稻的多个农艺性状和抗逆性。

Heterotrimeric G protein are involved in the regulation of multiple agronomic traits and stress tolerance in rice.

机构信息

Rice Research Institute of Shenyang Agricultural University, Shenyang, 110866, China.

Shenyang Research and Development Service Center of Modern Agriculture, Shenyang, 110866, China.

出版信息

BMC Plant Biol. 2020 Feb 28;20(1):90. doi: 10.1186/s12870-020-2289-6.

DOI:10.1186/s12870-020-2289-6
PMID:32111163
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7048073/
Abstract

BACKGROUND

The heterotrimeric G protein complex, consisting of Gα, Gβ, and Gγ subunits, are conserved signal transduction mechanism in eukaryotes. Recent molecular researches had demonstrated that G protein signaling participates in the regulation of yield related traits. However, the effects of G protein genes on yield components and stress tolerance are not well characterized.

RESULTS

In this study, we generated heterotrimeric G protein mutants in rice using CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) gene-editing technology. The effects of heterotrimeric G proteins on the regulation of yield components and stress tolerance were investigated. The mutants of gs3 and dep1 generated preferable agronomic traits compared to the wild-type, whereas the mutants of rga1 showed an extreme dwarf phenotype, which led to a dramatic decrease in grain production. The mutants showed improved stress tolerance, especially under salinity treatment. We found four putative extra-large G proteins (PXLG)1-4 that also participate in the regulation of yield components and stress tolerance. A yeast two hybrid showed that the RGB1 might interact with PXLG2 but not with PXLG1, PXLG3 or PXLG4.

CONCLUSION

These findings will not only improve our understanding of the repertoire of heterotrimeric G proteins in rice but also contribute to the application of heterotrimeric G proteins in rice breeding.

摘要

背景

三聚体 G 蛋白复合物由 Gα、Gβ 和 Gγ 亚基组成，是真核生物中保守的信号转导机制。最近的分子研究表明，G 蛋白信号参与了与产量相关性状的调节。然而，G 蛋白基因对产量构成和抗逆性的影响尚未得到很好的描述。

结果

本研究利用 CRISPR/Cas9（成簇规律间隔短回文重复）基因编辑技术在水稻中产生了三聚体 G 蛋白突变体。研究了三聚体 G 蛋白对产量构成和抗逆性的调节作用。与野生型相比，gs3 和 dep1 突变体产生了更好的农艺性状，而 rga1 突变体表现出极端的矮化表型，导致籽粒产量大幅下降。突变体表现出更好的抗逆性，尤其是在盐胁迫处理下。我们发现了四个可能的超大 G 蛋白（PXLG）1-4，它们也参与了产量构成和抗逆性的调节。酵母双杂交表明，RGB1 可能与 PXLG2 相互作用，但不与 PXLG1、PXLG3 或 PXLG4 相互作用。

结论

这些发现不仅将提高我们对水稻中三聚体 G 蛋白的认识，而且有助于在水稻育种中应用三聚体 G 蛋白。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ec/7048073/7f4116107348/12870_2020_2289_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ec/7048073/7ebd3e9dbb3b/12870_2020_2289_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ec/7048073/81cf7c8e2be3/12870_2020_2289_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ec/7048073/5bd0d7c3356b/12870_2020_2289_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ec/7048073/8b6ac753a77b/12870_2020_2289_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ec/7048073/1afad5be6a99/12870_2020_2289_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ec/7048073/5ba830bd93a5/12870_2020_2289_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ec/7048073/7f4116107348/12870_2020_2289_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ec/7048073/7ebd3e9dbb3b/12870_2020_2289_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ec/7048073/81cf7c8e2be3/12870_2020_2289_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ec/7048073/5bd0d7c3356b/12870_2020_2289_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ec/7048073/8b6ac753a77b/12870_2020_2289_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ec/7048073/1afad5be6a99/12870_2020_2289_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ec/7048073/5ba830bd93a5/12870_2020_2289_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ec/7048073/7f4116107348/12870_2020_2289_Fig7_HTML.jpg

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