LINC01354 通过上调整合素 β1 促进骨肉瘤细胞侵袭。

LINC01354 Promotes Osteosarcoma Cell Invasion by Up-regulating Integrin β1.

机构信息

Department of Bone Surgery, Daqing Oilfield General Hospital, Daqing, Heilongjiang Province, China.

Department of Ultrasound, Daqing Oilfield General Hospital, Daqing, Heilongjiang Province, China.

出版信息

Arch Med Res. 2020 Feb;51(2):115-123. doi: 10.1016/j.arcmed.2019.12.016. Epub 2020 Feb 25.

DOI:10.1016/j.arcmed.2019.12.016

PMID:32111497

Abstract

BACKGROUND AND AIMS

Long noncoding RNAs have been proved to play a key role in the development and progression of various tumors, including osteosarcoma (OS). However, the role and molecular mechanism of lncRNA in osteosarcoma metastasis remains unknown. Our purpose is to explore the clinical significance and biological function of LINC01354 in osteosarcoma.

METHODS

Expression of LINC01354 in OS tissues, serum and cell lines was measured and the association between LINC01354 expression and clinicopathological factors was analyzed. The functional effects of LINC01354 were examined in vitro by using transwell assays, western blot, immunohistochemistry (IHC) and in vivo in a xenograft tumor mouse model.

RESULTS

LINC01354 was overexpressed in OS tissues, serum and cells. LINC01354 overexpression promoted OS cells invasion, EMT and integrin β1 expression, while knockdown of LINC01354 inhibited OS cell invasion, epithelial-mesenchymal transition (EMT) and integrin β1 expression. In addition, integrin-β1 blockage with MAB13 antibody abrogated the effects of LINC01354 overexpression on promoting OS cells invasion and EMT. In addition, LINC01354 promoted OS cell metastasis in vivo.

CONCLUSION

LINC01354 promote OS cell EMT and invasion through up-regulating integrin β1. Our study suggested that LINC01354 may be regarded as a potential target for the clinical treatment of OS.

摘要

背景与目的

长链非编码 RNA 已被证明在各种肿瘤的发生和发展中发挥关键作用，包括骨肉瘤（OS）。然而，lncRNA 在骨肉瘤转移中的作用和分子机制尚不清楚。我们的目的是探讨 LINC01354 在骨肉瘤中的临床意义和生物学功能。

方法

检测 LINC01354 在 OS 组织、血清和细胞系中的表达，并分析 LINC01354 表达与临床病理因素的关系。通过 Transwell 测定、western blot、免疫组化（IHC）和异种移植肿瘤小鼠模型在体外研究 LINC01354 的功能效应。

结果

LINC01354 在 OS 组织、血清和细胞中高表达。LINC01354 过表达促进 OS 细胞侵袭、上皮间质转化（EMT）和整合素 β1 表达，而 LINC01354 敲低则抑制 OS 细胞侵袭、EMT 和整合素 β1 表达。此外，用 MAB13 抗体阻断整合素-β1 可消除 LINC01354 过表达对促进 OS 细胞侵袭和 EMT 的作用。此外，LINC01354 促进 OS 细胞体内转移。