巴西坚果摄入对他汀类药物使用者肌酸激酶活性、氧化应激和硒蛋白 mRNA 表达变化的影响的关联研究。

Association between creatine kinase activity, oxidative stress and selenoproteins mRNA expression changes after Brazil nut consumption of patients using statins.

机构信息

Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo - FMRP/USP, Brazil.

出版信息

Clin Nutr. 2020 Oct;39(10):3175-3181. doi: 10.1016/j.clnu.2020.02.012. Epub 2020 Feb 15.

DOI:10.1016/j.clnu.2020.02.012

Abstract

BACKGROUND & AIMS: Although the mechanisms by which statins promote muscle disorders remain unclear, supplementation with dietary antioxidants may mitigate statins' side effects. This study aimed to investigate whether the consumption of Brazil nuts modulates serum creatine kinase (CK) activity in patients regularly using statins.

METHODS

The study was performed in the Ribeirão Preto Medical School University Hospital. Thirty-two patients in regular use of statins were divided according to CK activity levels (G1: increased or G2: normal) and received one unit of Brazil nut daily for 3 months. Body composition, blood selenium (Se) concentrations, erythrocyte glutathione peroxidase (GPX) activity, oxidative stress parameters, and CK activity were evaluated before and after supplementation.

RESULTS

In both groups, supplementation with one Brazil nut daily for 3 months contributed to achieve decreased levels of CK activity in serum, with positive changes in plasma and erythrocyte Se concentrations (p < 0.0001), and increased levels of GPX activity. Among the parameters related to curbing of oxidative stress, we observed reduced levels of malondialdehyde (MDA) and superoxide dismutase (SOD) in both groups after supplementation. We also found a moderately negative association between CK and GPX activity (r = -41; p < 0.02). Expression of selenoproteins GPX1, SELENOP, and SELENON after Brazil nut supplementation was unchanged.

CONCLUSION

Brazil nut consumption enhanced the control of CK activity by improving oxidative stress biomarkers in patients using statins but did not modulate mRNA expression of selenoproteins.

摘要

背景与目的

尽管他汀类药物促进肌肉疾病的机制尚不清楚，但膳食抗氧化剂的补充可能减轻他汀类药物的副作用。本研究旨在探讨食用巴西坚果是否会调节经常使用他汀类药物的患者的血清肌酸激酶（CK）活性。

方法

该研究在里贝朗普雷图医学院附属医院进行。32 名经常使用他汀类药物的患者根据 CK 活性水平（G1：升高或 G2：正常）进行分组，并在 3 个月内每天接受一个巴西坚果。补充前后评估身体成分、血液硒（Se）浓度、红细胞谷胱甘肽过氧化物酶（GPX）活性、氧化应激参数和 CK 活性。

结果

在两组中，每天补充一个巴西坚果 3 个月有助于降低血清 CK 活性水平，血浆和红细胞 Se 浓度呈正变化（p<0.0001），GPX 活性增加。在抑制氧化应激的参数中，我们观察到补充后两组 MDA 和 SOD 水平降低。补充后 CK 和 GPX 活性之间存在中度负相关（r=-41；p<0.02）。巴西坚果补充后，硒蛋白 GPX1、SELENOP 和 SELENON 的表达没有变化。