Niktalab Rana, Piravar Zeinab, Behzadi Roudabeh
Department of Biology, Faculty of Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Department of Biology, Faculty of Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran. Electronic Address:
Int J Fertil Steril. 2020 Apr;14(1):41-45. doi: 10.22074/ijfs.2020.5787. Epub 2020 Feb 25.
Pre-eclampsia (PE) is a pregnancy complication and one of the leading causes of maternal and neonatal morbidity and mortality in the world. PE is characterized by high blood pressure and signs of damage to the other organs, most often the liver and kidneys. Given the importance of mutation in the vascular endothelial growth factor () gene and its correlation with the incidence of PE, the relationship of encoding gene polymorphisms rs922583280, rs3025040 and rs10434 with the incidence of PE in the population of Iranian women was studied, in this research.
In this case-control study, 100 pregnant women with PE diagnosis and 50 healthy pregnant women were evaluated using Sanger sequencing method to determine genotypes rs922583280, rs3025040 and rs10434.
There was no significant difference in the allele frequency of rs922583280 and rs3025040 polymorphisms between case and control groups (P>0.05), while frequency of the recessive allele (G) for rs10434 polymorphism was significantly higher in the case group compared to the control group (P=0.014, case=24%, control=12%). Frequency of the allele A in the control group was higher than the patient group (case=76%, control=88%). Frequency of AG genotype in the patient group was also higher than the control group. In addition, frequency of AA genotype in the control group was higher than the patient group (case=57%, control=78).
The results of this study demonstrated a significant difference between patient and control groups for the coding gene polymorphism rs10434 and it can affect the incidence of PE among Iranian women.
子痫前期（PE）是一种妊娠并发症，是全球孕产妇和新生儿发病及死亡的主要原因之一。PE的特征是高血压以及其他器官（最常见的是肝脏和肾脏）受损的迹象。鉴于血管内皮生长因子（）基因突变的重要性及其与PE发病率的相关性，本研究探讨了编码基因多态性rs922583280、rs3025040和rs10434与伊朗女性人群中PE发病率的关系。
在这项病例对照研究中，采用桑格测序法对100例诊断为PE的孕妇和50例健康孕妇进行评估，以确定rs922583280、rs3025040和rs10434的基因型。
病例组和对照组之间rs922583280和rs3025040多态性的等位基因频率无显著差异（P>0.05），而rs10434多态性的隐性等位基因（G）频率在病例组中显著高于对照组（P=0.014，病例组=24%，对照组=12%）。对照组中A等位基因的频率高于患者组（病例组=76%，对照组=88%）。患者组中AG基因型的频率也高于对照组。此外，对照组中AA基因型的频率高于患者组（病例组=57%，对照组=78）。
本研究结果表明，病例组和对照组在编码基因多态性rs10434方面存在显著差异，它可能影响伊朗女性中PE的发病率。
