Salimi Saeedeh, Yaghmaei Minoo, Tabatabaei Ehsan, Mokhtari Mojgan, Naghavi Anoosh
Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
J Obstet Gynaecol Res. 2015 Dec;41(12):1877-83. doi: 10.1111/jog.12825. Epub 2015 Oct 21.
Vascular endothelial growth factor (VEGF) is an angiogenic factor whose production is increased in pre-eclampsia (PE). Therefore, the present study was conducted aiming at assessing the possible association of VEGF polymorphisms with PE susceptibility in the southeast of Iran.
Overall, 192 PE women and 186 unrelated age-matched normotensive pregnant women were genotyped for the VEGF-2578C/A (rs699947), -1154G/A (rs1570360), and -634G/C (rs2010963) polymorphisms using the polymerase chain reaction-restriction fragment length polymorphism method. Serum VEGF levels were determined by the enzyme-linked immunosorbent assay method.
There was no significant difference in VEGF-2578C/A, -1154G/A and -634G/C polymorphisms between PE women and controls. However, the frequency of VEGF-634GC and CC genotypes was significantly higher in women with severe PE compared to mild PE and controls. In addition, serum VEGF levels were significantly lower in PE women. The VEGF-634CC genotype was associated with lower serum VEGF levels compared to the VEGF-634GG genotype. Moreover, serum VEGF levels were significantly lower in individuals with the VEGF-634CC genotype compared to VEGF-634GC genotype only in the control group. The mean serum VEGF levels did not differ significantly between genotypes of VEGF-2587C/A and -1154G/A polymorphisms.
Our findings suggest that the association of VEGF-634G/C polymorphisms with severe PE and the VEGF-634CC genotype was correlated with lower serum VEGF levels.
血管内皮生长因子（VEGF）是一种血管生成因子，其在子痫前期（PE）中产量增加。因此，本研究旨在评估伊朗东南部VEGF基因多态性与PE易感性之间的可能关联。
总体而言，采用聚合酶链反应-限制性片段长度多态性方法对192例PE女性和186例年龄匹配的无亲缘关系的血压正常孕妇进行VEGF - 2578C/A（rs699947）、-1154G/A（rs1570360）和-634G/C（rs2010963）基因多态性基因分型。采用酶联免疫吸附测定法测定血清VEGF水平。
PE女性与对照组在VEGF - 2578C/A、-1154G/A和-634G/C基因多态性方面无显著差异。然而，与轻度PE和对照组相比，重度PE女性中VEGF - 634GC和CC基因型的频率显著更高。此外，PE女性的血清VEGF水平显著更低。与VEGF - 634GG基因型相比，VEGF - 634CC基因型与更低的血清VEGF水平相关。此外，仅在对照组中，VEGF - 634CC基因型个体的血清VEGF水平显著低于VEGF - 634GC基因型个体。VEGF - 2587C/A和-1154G/A基因多态性各基因型之间的平均血清VEGF水平无显著差异。
我们的研究结果表明，VEGF - 634G/C基因多态性与重度PE的关联以及VEGF - 634CC基因型与更低的血清VEGF水平相关。
