Korakaki Dimitra, Mouroutsos Spyros, Tripsianis Gregory, Nikolettos Nikos, Asimakopoulos Byron
Laboratory of Physiology, Faculty of Medicine, School of Health Sciences, Democritus University of Thrace, Alexandroupolis, Greece.
Laboratory of Mechatronics and Systems Automation, Department of Electrical and Computer Engineering, Democritus University of Thrace, Xanthi, Greece.
Int J Fertil Steril. 2020 Apr;14(1):63-67. doi: 10.22074/ijfs.2020.5917. Epub 2020 Feb 25.
In embryological culture dishes, there is a temperature decline when they are removed outside incubators. This study aimed at investigating the effects of this temperature decline within a certain time frame, the type of culture dish with or without the use of laminar air flow and whether it is possible to achieve a sufficient thermal control with the use of a heating stage.
In this experimental study, the temperatures of four different types of polystyrene dishes [50 mm intracytoplasmic sperm injection (ICSI), 35 mm, 60 mm, 90 mm], filled with culture medium and oil were recorded for a period of 10 minutes outside the incubator. Temperature was measured with an infrared thermographic camera. The reference temperature was 37°C. Four parameters were analyzed: the type of dishes, air flow, a heating stage at 37°C and 38.5°C.
There was a time-dependant significant temperature decline outside the incubator in all types of dishes and under all experimental conditions. Under air flow temperature decline increased compared to the no air flow condition. The use of a heating stage at either 37°C or 38.5°C slightly improved the situation in most cases. After three minutes out of the incubator without a heating stage and air flow, the temperature was <34°C; with air flow and without a heating stage the temperature was <33°C. When a heating stage was used, the temperature was <36°C, except when using ICSI dishes. When ICSI dishes were on a heating stage they maintained a temperature close to 37°C with or without air flow. In all experimental conditions the highest decline was recorded with the 90 mm dishes.
Time is crucial for managing the temperature decline in culture dishes when out of the incubator. Under air laminar flow, the heat loss is greater, when with a heating stage at 37°C or better at 38.5°C this loss decreases but still exists. ICSI flat bottom dishes give the best results when heated stages are used. Flat bottom dishes maintain the temperature rather efficiently. Based on our findings, the use of flat bottom dishes should become a universal practice in fertilization (IVF).
在胚胎培养皿从培养箱中取出时，其温度会下降。本研究旨在调查在一定时间范围内这种温度下降的影响、使用或不使用层流的培养皿类型，以及使用加热台是否能够实现充分的温度控制。
在本实验研究中，使用红外热成像相机记录了四种不同类型的聚苯乙烯培养皿（50mm 卵胞浆内单精子注射[ICSI]培养皿、35mm 培养皿、60mm 培养皿、90mm 培养皿），在装有培养基和油的情况下，于培养箱外放置 10 分钟期间的温度。参考温度为 37°C。分析了四个参数：培养皿类型、气流、37°C 和 38.5°C 的加热台。
在所有类型的培养皿以及所有实验条件下，培养箱外的温度均随时间显著下降。与无气流条件相比，在有气流时温度下降幅度更大。在大多数情况下，使用 37°C 或 38.5°C 的加热台可使情况稍有改善。在无加热台且无气流的情况下，从培养箱中取出三分钟后，温度低于 34°C；有气流但无加热台时，温度低于 33°C。当使用加热台时，除了 ICSI 培养皿外，温度均低于 36°C。当 ICSI 培养皿置于加热台上时，无论有无气流，其温度均能保持接近 37°C。在所有实验条件下，90mm 培养皿的温度下降幅度最大。
对于管理培养皿从培养箱中取出时的温度下降，时间至关重要。在层流空气条件下，热损失更大，当使用 37°C 或更好的 38.5°C 加热台时，这种损失会减少但仍然存在。当使用加热台时，ICSI 平底培养皿效果最佳。平底培养皿能相当有效地保持温度。基于我们的研究结果，在体外受精（IVF）中使用平底培养皿应成为普遍做法。
