Department of Polymer Engineering, Shahreza Branch, Islamic Azad University, Shahreza, Iran.
Department of Polymer Engineering, Shahreza Branch, Islamic Azad University, Shahreza, Iran.
Int J Biol Macromol. 2019 Jul 15;133:881-891. doi: 10.1016/j.ijbiomac.2019.04.159. Epub 2019 Apr 24.
In order to improve mechanical properties and biomedical behavior of chitosan-based polymeric films, the effect of nanocrystalline cellulose (NCC) and calcium peroxide (CP) particles on the properties of polymeric films based on chitosan (CS) and gelatin (GL) were investigated in this study. The films were characterized by Fourier-transform infrared (FTIR), tensile, swelling, water vapor transmission rate (WVTR), antibacterial, oxygen release and cell culture tests. FTIR results indicated that hydrogen bonding has been formed between functional groups of the constituents. The mechanical results showed that the combination of both CP and NCC had better results in improving the mechanical properties of the films. The WVTR and swelling results indicated that CP and NCC particles reduced the amount of WVTR and swelling of the samples. By Adding CP to the film composition, the antibacterial activity of the films against E. coli bacteria increased. The oxygen release for the films containing CP has its maximum value during the first day and it approaches a constant value for 10 days. The MTT assay results revealed that the growth of the human fibroblast cells was increased during 7 days, showing that the chitosan-based films containing CP and NCC had no toxicity and never cause cell death.
为了提高壳聚糖基聚合物薄膜的机械性能和生物医学性能，本研究考察了纳米晶纤维素（NCC）和过氧化钙（CP）颗粒对壳聚糖（CS）和明胶（GL）基聚合物薄膜性能的影响。采用傅里叶变换红外（FTIR）、拉伸、溶胀、水蒸气透过率（WVTR）、抗菌、氧释放和细胞培养试验对薄膜进行了表征。FTIR 结果表明，各组成成分的官能团之间形成了氢键。力学性能结果表明，CP 和 NCC 的结合在改善薄膜的力学性能方面具有更好的效果。WVTR 和溶胀结果表明 CP 和 NCC 颗粒减少了样品的 WVTR 和溶胀量。通过向薄膜组成中添加 CP，薄膜对大肠杆菌的抗菌活性增加。含 CP 的薄膜的氧释放在第一天达到最大值，然后在 10 天内接近恒定值。MTT 试验结果表明，人成纤维细胞在 7 天内的生长增加，表明含有 CP 和 NCC 的壳聚糖基薄膜没有毒性，从不导致细胞死亡。
