College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China; Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource, Nanjing Agricultural University, Nanjing 210095, China.
Sci Total Environ. 2020 Jun 1;719:137475. doi: 10.1016/j.scitotenv.2020.137475. Epub 2020 Feb 20.
To explore the potential of ectomycorrhizal (ECM) Pinus sylvestris (P. sylvestris) utilizing in the phytoremediation of a combined heavy metal contaminated tailings pond, Pisolithus sp.1(P1)-. Pisolithus sp.2 (P2)-. Cenococcum geophilum (Cg)-. Laccaria sp. (L1)- ECM, and non-ectomycorrhizal (NM) P. sylvestris were planted separately in lead (Pb)-zinc-(Zn)-cadmium-(Cd)-combined polluted soil, collected from a tailings pond. After four months, growth, photosynthetic parameters, nutrient and heavy metal levels of the plants were evaluated. The physical and chemical properties and enzyme activities of soil before and after ECM plants planting were also investigated. The results showed that inoculation with ECM fungi improved the survival rates of host plants by increasing the biomass, photosynthesis (photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), intercellular CO concentration (Ci)), and mineral nutrients (phosphorus (Pi), magnesium (Mg), iron (Fe), calcium (Ca)), while it decreased the transfer factors of Cd, Pb, and Zn. In addition, ECM P. sylvestris significantly accumulated much more Cd, Pb, and Zn than NM seedlings, while it reduced pH and the availability of heavy metals (DTPA-Cd, DTPA-Pb, DTPA-Zn) in soil and increased activity of soil enzymes (acid phosphatase, alkaline phosphatase, urease). Therefore, the ECM symbionts have the great potential for phytoremediation of abandoned tailings pond, and this study provides a theoretical basis and application premise for the phytoremediation of abandoned tailings pond.
为了探索外生菌根（ECM）欧洲赤松（P. sylvestris）在重金属复合污染尾矿库植物修复中的潜力，分别在来自尾矿库的铅（Pb）-锌（Zn）-镉（Cd）-复合污染土壤中种植了外生菌根（ECM）欧洲赤松（P. sylvestris）与非外生菌根（NM）欧洲赤松，并接种了丛枝菌根真菌（Pisolithus sp.1（P1）-，Pisolithus sp.2（P2）-，Cenococcum geophilum（Cg）-，Laccaria sp.（L1）-）。四个月后，评估了植物的生长、光合参数、养分和重金属水平。还研究了 ECM 植物种植前后土壤的物理和化学性质以及酶活性。结果表明，接种 ECM 真菌通过增加生物量、光合作用（光合速率（Pn）、气孔导度（Gs）、蒸腾速率（Tr）、胞间 CO 浓度（Ci））和矿物质养分（磷（Pi）、镁（Mg）、铁（Fe）、钙（Ca））来提高宿主植物的存活率，同时降低 Cd、Pb 和 Zn 的转移因子。此外，与 NM 幼苗相比，ECM 欧洲赤松显著积累了更多的 Cd、Pb 和 Zn，同时降低了土壤的 pH 值和重金属的有效性（DTPA-Cd、DTPA-Pb、DTPA-Zn），并增加了土壤酶（酸性磷酸酶、碱性磷酸酶、脲酶）的活性。因此，ECM 共生体在外生菌根废弃尾矿库植物修复中具有巨大的潜力，本研究为废弃尾矿库的植物修复提供了理论依据和应用前提。
