Yokoyama Shin-Ichiro, Kodera Miyuki, Hirai Akiko, Nakada Mitsuhiko, Ueno Yuki, Osawa Toshihiko
Gifu Prefectural Research Institute for Food Sciences.
Saladcosmo Co. Ltd.
J Nutr Sci Vitaminol (Tokyo). 2020;66(1):48-53. doi: 10.3177/jnsv.66.48.
We examined the prevention effect of red clover (Trifolium pratense L.) sprout on metabolic syndrome using a high-carbohydrate and high-fat diet (Western diet; WD)-induced male C57BL/6J obese model mouse. Red clover sprout-lyophilized powder (RC) contained 3.5 mg/g dry-weight of formononetin as a major phenolic compound, as analyzed by high performance liquid chromatography. Supplementation of 0.3% (w/w) RC in a WD (WD+RC) showed an anti-obesity effect and ameliorated lipid metabolism in the obese model mice. Additionally, fasting plasma glucose levels were significantly reduced in the WD+RC group. Administration of 0.1 mg/kg formononetin reduced the postprandial blood glucose level, as assessed using the oral maltose tolerance test. However, no significant formononetin intake effect was observed on the plasma insulin level. These results suggest that the formononetin contained in red clover sprout inhibits α-glucosidase and thereby contributes to reducing the postprandial blood glucose response in mice.
我们使用高碳水化合物高脂肪饮食（西方饮食；WD）诱导的雄性C57BL/6J肥胖模型小鼠，研究了红三叶草（Trifolium pratense L.）芽苗对代谢综合征的预防作用。通过高效液相色谱分析，红三叶草芽苗冻干粉末（RC）含有3.5毫克/克干重的芒柄花素作为主要酚类化合物。在WD中添加0.3%（w/w）的RC（WD+RC）对肥胖模型小鼠显示出抗肥胖作用并改善了脂质代谢。此外，WD+RC组的空腹血糖水平显著降低。使用口服麦芽糖耐量试验评估，给予0.1毫克/千克芒柄花素可降低餐后血糖水平。然而，未观察到芒柄花素摄入对血浆胰岛素水平有显著影响。这些结果表明，红三叶草芽苗中含有的芒柄花素抑制α-葡萄糖苷酶，从而有助于降低小鼠餐后血糖反应。
