Hoshino Kiyoto, Kugo Hirona, Miyamoto Chie, Hashimoto Keisuke, Murase Hiroshi, Mizuno Masatoshi, Moriyama Tatsuya, Zaima Nobuhiro
Department of Applied Biological Chemistry, Graduate School of Agriculture, Kindai University.
Otsuka Foods Co., Ltd. Biwako Research Institute.
J Nutr Sci Vitaminol (Tokyo). 2020;66(1):75-81. doi: 10.3177/jnsv.66.75.
Abdominal aortic aneurysm (AAA) is a vascular disease characterized by weakening of vascular walls and progressive dilation of the abdominal aorta. Nicotine, the main component of tobacco, is reportedly associated with the development and rupture of AAA. It is desirable to attenuate the destructive effect of nicotine on vascular walls, using dietary food components. However, effective methods for preventing AAA progression using dietary food components remain unestablished. This study focuses on proanthocyanidins, well known for their potent antioxidant activity. We speculated that proanthocyanidins can suppress nicotine-induced weakening of vascular walls. To estimate the effect of black soybean seed coat extract (BSSCE), rich in proanthocyanidins, on nicotine-induced weakening of the aortic wall, mice were divided into four groups: the control diet and distilled water group (named C), BSSCE solution diet and distilled water group (named B), control diet and 0.5 mg/mL nicotine solution group (named CN), and BSSCE solution diet and 0.5 mg/mL nicotine solution group (named BN). Nicotine-induced degradation of elastin and collagen fibers were significantly suppressed in BN group. The positive areas for matrix metalloproteinase (MMP)-2 and oxidative stress in BN group were significantly decreased compared to those in CN group. These results suggest that proanthocyanidins-rich BSSCE can prevent the weakening of the aortic wall via inhibiting MMP-2 upregulation.
腹主动脉瘤（AAA）是一种血管疾病，其特征是血管壁变薄和腹主动脉逐渐扩张。据报道，烟草的主要成分尼古丁与AAA的发生和破裂有关。使用膳食成分来减轻尼古丁对血管壁的破坏作用是可取的。然而，利用膳食成分预防AAA进展的有效方法尚未确立。本研究聚焦于原花青素，其以强大的抗氧化活性而闻名。我们推测原花青素可以抑制尼古丁诱导的血管壁变薄。为了评估富含原花青素的黑豆种皮提取物（BSSCE）对尼古丁诱导的主动脉壁变薄的影响，将小鼠分为四组：对照饮食和蒸馏水组（命名为C）、BSSCE溶液饮食和蒸馏水组（命名为B）、对照饮食和0.5 mg/mL尼古丁溶液组（命名为CN）以及BSSCE溶液饮食和0.5 mg/mL尼古丁溶液组（命名为BN）。在BN组中，尼古丁诱导的弹性蛋白和胶原纤维降解受到显著抑制。与CN组相比，BN组基质金属蛋白酶（MMP）-2的阳性区域和氧化应激显著降低。这些结果表明，富含原花青素的BSSCE可通过抑制MMP-2上调来预防主动脉壁变薄。
