The Full-Size ABCG Transporter of Is Involved in Strigolactone Secretion, Affecting Arbuscular Mycorrhiza.

Strigolactones (SLs) are plant-derived signaling molecules that stimulate the hyphal branching of arbuscular mycorrhizal fungi (AMF), and consequently promote symbiotic interaction between the fungus and the plant. Currently, our knowledge on the molecular mechanism of SL transport is restricted to the family. In the family, SL translocation toward the rhizosphere occurs through the exodermis hypodermal passage cells and involves a member of the G subfamily, of the ATP-binding cassette (ABC) membrane transporters. Most species, including those that are agriculturally important, have a different root anatomy compared to most angiosperm plants (i.e., lacking an exodermis). Thus, we have investigated how SL transport occurs in the model legume . Here, we show that overexpression of a SL transporter from petunia (PaPDR1) enhances AMF colonization rates in . This result demonstrates the importance of ABCG proteins for the translocation of orobanchol-type molecules to facilitate arbuscular mycorrhiza, regardless of root anatomy and phylogenetic relationships. Moreover, our research has led to the identification of Medicago ABCG59, a close homologue of Petunia PDR1, that exhibits root specific expression and is up-regulated by phosphate starvation as well as in the presence of -GR24, a synthetic SL. Its promoter is active in cortical cells, root tips, and the meristematic zone of nodules. The loss-of-function mutant displayed a reduced level of mycorrhization compared to the WT plants but had no impact on the number of nodules after inoculation. The reduced mycorrhization indicates that less SLs are secreted by the mutant plants, which is in line with the observation that exudates exhibit a reduced stimulatory effect on the germination of the parasitic plant compared to the corresponding wild type.