• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

高维质谱流式细胞术分析揭示弥漫性星形细胞瘤免疫抑制微环境的特征

High Dimensional Mass Cytometry Analysis Reveals Characteristics of the Immunosuppressive Microenvironment in Diffuse Astrocytomas.

作者信息

Fu Weilun, Wang Wenjing, Li Hao, Jiao Yuming, Weng Jiancong, Huo Ran, Yan Zihan, Wang Jie, Xu Hongyuan, Wang Shuo, Wang Jiangfei, Chen Dexi, Cao Yong, Zhao Jizong

机构信息

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

China National Clinical Research Center for Neurological Diseases, Beijing, China.

出版信息

Front Oncol. 2020 Feb 4;10:78. doi: 10.3389/fonc.2020.00078. eCollection 2020.

DOI:10.3389/fonc.2020.00078
PMID:32117733
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7010913/
Abstract

The tumor immune microenvironment (TIME) plays a pivotal role in tumor development, progression, and prognosis. However, the characteristics of the TIME in diffuse astrocytoma (DA) are still unclear. Leveraging mass cytometry with a panel of 33 markers, we analyzed the infiltrating immune cells from 10 DA and 4 oligodendroglioma (OG) tissues and provided a single cell-resolution landscape of the intricate immune microenvironment. Our study profiled the composition of the TIME in DA and confirmed the presence of immune cells, such as glioma-associated microglia/macrophages (GAMs), CD8+ T cells, CD4+ T cells, regulatory T cells (Tregs), and natural killer cells. Increased percentages of PD-1+ CD8+ T cells, TIM-3+ CD4+ T cell subpopulations, Tregs and pro-tumor phenotype GAMs substantially contribute to the local immunosuppressive microenvironment in DA. DAs and OGs share similar compositions in terms of immune cells, while GAMs in DA exhibit more inhibitory characteristics than those in OG.

摘要

肿瘤免疫微环境（TIME）在肿瘤的发生、发展和预后中起着关键作用。然而，弥漫性星形细胞瘤（DA）中TIME的特征仍不清楚。我们利用包含33种标志物的质谱流式细胞术，分析了10例DA组织和4例少突胶质细胞瘤（OG）组织中的浸润免疫细胞，并提供了复杂免疫微环境的单细胞分辨率图谱。我们的研究描绘了DA中TIME的组成，并证实了免疫细胞的存在, 如胶质瘤相关小胶质细胞/巨噬细胞（GAMs）、CD8 + T细胞、CD4 + T细胞、调节性T细胞（Tregs）和自然杀伤细胞。PD-1 + CD8 + T细胞、TIM-3 + CD4 + T细胞亚群、Tregs和促肿瘤表型GAMs百分比的增加，在很大程度上促成了DA中的局部免疫抑制微环境。DA和OG在免疫细胞组成方面相似，而DA中的GAMs比OG中的GAMs表现出更多的抑制特征。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f34e/7010913/168c0f414f49/fonc-10-00078-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f34e/7010913/caacd90c9865/fonc-10-00078-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f34e/7010913/d94cfa25fca4/fonc-10-00078-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f34e/7010913/5c127740101d/fonc-10-00078-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f34e/7010913/a1c277a54e31/fonc-10-00078-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f34e/7010913/9522b45190f2/fonc-10-00078-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f34e/7010913/168c0f414f49/fonc-10-00078-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f34e/7010913/caacd90c9865/fonc-10-00078-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f34e/7010913/d94cfa25fca4/fonc-10-00078-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f34e/7010913/5c127740101d/fonc-10-00078-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f34e/7010913/a1c277a54e31/fonc-10-00078-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f34e/7010913/9522b45190f2/fonc-10-00078-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f34e/7010913/168c0f414f49/fonc-10-00078-g0006.jpg

相似文献

1
High Dimensional Mass Cytometry Analysis Reveals Characteristics of the Immunosuppressive Microenvironment in Diffuse Astrocytomas.高维质谱流式细胞术分析揭示弥漫性星形细胞瘤免疫抑制微环境的特征
Front Oncol. 2020 Feb 4;10:78. doi: 10.3389/fonc.2020.00078. eCollection 2020.
2
Single-Cell Atlas Reveals Complexity of the Immunosuppressive Microenvironment of Initial and Recurrent Glioblastoma.单细胞图谱揭示初始和复发性脑胶质瘤免疫抑制微环境的复杂性。
Front Immunol. 2020 May 7;11:835. doi: 10.3389/fimmu.2020.00835. eCollection 2020.
3
CyTOF Analysis Reveals a Distinct Immunosuppressive Microenvironment in IDH Mutant Anaplastic Gliomas.质谱流式细胞术分析揭示了异柠檬酸脱氢酶（IDH）突变型间变性胶质瘤中独特的免疫抑制微环境。
Front Oncol. 2021 Feb 4;10:560211. doi: 10.3389/fonc.2020.560211. eCollection 2020.
4
Delineation of an immunosuppressive gradient in hepatocellular carcinoma using high-dimensional proteomic and transcriptomic analyses.利用高维蛋白质组学和转录组学分析描绘肝细胞癌中的免疫抑制梯度。
Proc Natl Acad Sci U S A. 2017 Jul 18;114(29):E5900-E5909. doi: 10.1073/pnas.1706559114. Epub 2017 Jul 3.
5
Stromal PD-L1-Positive Regulatory T cells and PD-1-Positive CD8-Positive T cells Define the Response of Different Subsets of Non-Small Cell Lung Cancer to PD-1/PD-L1 Blockade Immunotherapy.基质 PD-L1 阳性调节性 T 细胞和 PD-1 阳性 CD8 阳性 T 细胞定义了不同亚组非小细胞肺癌对 PD-1/PD-L1 阻断免疫治疗的反应。
J Thorac Oncol. 2018 Apr;13(4):521-532. doi: 10.1016/j.jtho.2017.11.132. Epub 2017 Dec 18.
6
Identification of clinically relevant subsets CD39PD-1CD8 T cells and CD39 regulatory T cells in intrahepatic cholangiocarcinoma using single-cell CyTOF.使用单细胞质谱流式细胞术鉴定肝内胆管癌中临床相关的亚群CD39⁺PD-1⁺CD8⁺ T细胞和CD39⁺调节性T细胞
Transl Oncol. 2024 Jun;44:101954. doi: 10.1016/j.tranon.2024.101954. Epub 2024 Apr 11.
7
Enhanced immunity in a mouse model of malignant glioma is mediated by a therapeutic ketogenic diet.治疗性生酮饮食可介导恶性胶质瘤小鼠模型的免疫增强。
BMC Cancer. 2016 May 13;16:310. doi: 10.1186/s12885-016-2337-7.
8
Immune Profiling and Quantitative Analysis Decipher the Clinical Role of Immune-Checkpoint Expression in the Tumor Immune Microenvironment of DLBCL.免疫分析和定量分析解析免疫检查点表达在 DLBCL 肿瘤免疫微环境中的临床作用。
Cancer Immunol Res. 2019 Apr;7(4):644-657. doi: 10.1158/2326-6066.CIR-18-0439. Epub 2019 Feb 11.
9
Pro-inflammatory chemokine-chemokine receptor interactions within the Ewing sarcoma microenvironment determine CD8(+) T-lymphocyte infiltration and affect tumour progression.促炎趋化因子-趋化因子受体相互作用在尤文肉瘤微环境中决定 CD8(+) T 淋巴细胞浸润并影响肿瘤进展。
J Pathol. 2011 Feb;223(3):347-57. doi: 10.1002/path.2819. Epub 2010 Dec 10.
10
Clinicopathological features in the recurrence of oligodendroglioma and diffuse astrocytoma.少突胶质细胞瘤和弥漫性星形细胞瘤复发的临床病理特征。
Brain Tumor Pathol. 2012 Jul;29(3):140-7. doi: 10.1007/s10014-012-0104-2. Epub 2012 May 31.

引用本文的文献

1
ceRNA network-regulated COL1A2 high expression correlates with poor prognosis and immune infiltration in colon adenocarcinoma.环状 RNA 调控网络调控 COL1A2 高表达与结直肠腺癌不良预后和免疫浸润相关。
Sci Rep. 2023 Oct 7;13(1):16932. doi: 10.1038/s41598-023-43507-x.
2
Tumor Microenvironment in Gliomas: A Treatment Hurdle or an Opportunity to Grab?胶质瘤中的肿瘤微环境：治疗障碍还是可把握的机遇？
Cancers (Basel). 2023 Feb 7;15(4):1042. doi: 10.3390/cancers15041042.
3
Tumor-Associated Microenvironment of Adult Gliomas: A Review.成人胶质瘤的肿瘤相关微环境：综述

本文引用的文献

1
The current state of immunotherapy for gliomas: an eye toward the future.当前胶质母细胞瘤的免疫治疗现状：着眼于未来。
J Neurosurg. 2019 Sep 1;131(3):657-666. doi: 10.3171/2019.5.JNS181762.
2
Effect of Treatment Modalities on Progression-Free Survival and Overall Survival in Molecularly Subtyped World Health Organization Grade II Diffuse Gliomas: A Systematic Review.治疗方式对分子亚型世界卫生组织分级 II 弥漫性神经胶质瘤无进展生存期和总生存期的影响：系统评价。
World Neurosurg. 2020 Jan;133:366-380.e2. doi: 10.1016/j.wneu.2019.08.111. Epub 2019 Aug 29.
3
The tumor immune microenvironment in gastroenteropancreatic neuroendocrine neoplasms.
Front Oncol. 2022 Jul 7;12:891543. doi: 10.3389/fonc.2022.891543. eCollection 2022.
4
Glioma-Associated Microglia Characterization in the Glioblastoma Microenvironment through a 'Seed-and Soil' Approach: A Systematic Review.通过“种子与土壤”方法对胶质母细胞瘤微环境中胶质瘤相关小胶质细胞的特征进行系统评价
Brain Sci. 2022 May 31;12(6):718. doi: 10.3390/brainsci12060718.
5
Gene Expression-Based Predication of RNA Pseudouridine Modification in Tumor Microenvironment and Prognosis of Glioma Patients.基于基因表达预测肿瘤微环境中的RNA假尿苷修饰及胶质瘤患者的预后
Front Cell Dev Biol. 2022 Jan 18;9:727595. doi: 10.3389/fcell.2021.727595. eCollection 2021.
6
Single-Cell Omics in Dissecting Immune Microenvironment of Malignant Gliomas-Challenges and Perspectives.单细胞组学解析恶性脑胶质瘤免疫微环境：挑战与展望。
Cells. 2021 Aug 31;10(9):2264. doi: 10.3390/cells10092264.
7
Role of Temozolomide Regimen on Survival Outcomes in Molecularly Stratified WHO Grade II Gliomas: A Systematic Review.替莫唑胺方案对分子分层的世界卫生组织二级胶质瘤生存结果的作用：一项系统评价
Asian J Neurosurg. 2021 Feb 23;16(1):14-23. doi: 10.4103/ajns.AJNS_186_20. eCollection 2021 Jan-Mar.
8
Putting Proteomics Into Immunotherapy for Glioblastoma.将蛋白质组学应用于胶质母细胞瘤的免疫治疗。
Front Immunol. 2021 Feb 23;12:593255. doi: 10.3389/fimmu.2021.593255. eCollection 2021.
9
CyTOF Analysis Reveals a Distinct Immunosuppressive Microenvironment in IDH Mutant Anaplastic Gliomas.质谱流式细胞术分析揭示了异柠檬酸脱氢酶（IDH）突变型间变性胶质瘤中独特的免疫抑制微环境。
Front Oncol. 2021 Feb 4;10:560211. doi: 10.3389/fonc.2020.560211. eCollection 2020.
10
The Past, Present and Future of Flow Cytometry in Central Nervous System Malignancies.流式细胞术在中枢神经系统恶性肿瘤中的过去、现在与未来
Methods Protoc. 2021 Jan 26;4(1):11. doi: 10.3390/mps4010011.
胃肠胰神经内分泌肿瘤的肿瘤免疫微环境。
Biochim Biophys Acta Rev Cancer. 2019 Dec;1872(2):188311. doi: 10.1016/j.bbcan.2019.188311. Epub 2019 Aug 20.
4
Monitoring Patient Response to Pembrolizumab With Peripheral Blood Exhaustion Marker Profiles.通过外周血耗竭标志物谱监测患者对帕博利珠单抗的反应。
Front Med (Lausanne). 2019 May 22;6:113. doi: 10.3389/fmed.2019.00113. eCollection 2019.
5
Tumor-associated macrophages: role in cancer development and therapeutic implications.肿瘤相关巨噬细胞：在癌症发展中的作用及治疗意义。
Cell Oncol (Dordr). 2019 Oct;42(5):591-608. doi: 10.1007/s13402-019-00453-z. Epub 2019 May 29.
6
The landscape of postsurgical recurrence patterns in diffuse low-grade gliomas.弥漫性低级别胶质瘤术后复发模式的研究现状。
Crit Rev Oncol Hematol. 2019 Jun;138:148-155. doi: 10.1016/j.critrevonc.2019.04.009. Epub 2019 Apr 15.
7
Lipopolysaccharide inhalation recruits monocytes and dendritic cell subsets to the alveolar airspace.脂多糖吸入可招募单核细胞和树突状细胞亚群至肺泡腔。
Nat Commun. 2019 Apr 30;10(1):1999. doi: 10.1038/s41467-019-09913-4.
8
CD8 T cell exhaustion.CD8 T 细胞耗竭。
Semin Immunopathol. 2019 May;41(3):327-337. doi: 10.1007/s00281-019-00744-5. Epub 2019 Apr 15.
9
Interaction of Breast Cancer and Insulin Resistance on PD1 and TIM3 Expression in Peripheral Blood CD8 T Cells.乳腺癌与胰岛素抵抗对外周血 CD8 T 细胞 PD1 和 TIM3 表达的相互作用。
Pathol Oncol Res. 2019 Jul;25(3):1233-1243. doi: 10.1007/s12253-019-00610-7. Epub 2019 Feb 13.
10
Mass cytometry: a powerful tool for dissecting the immune landscape.质谱流式细胞术：解析免疫图谱的强大工具。
Curr Opin Immunol. 2018 Apr;51:187-196. doi: 10.1016/j.coi.2018.03.023. Epub 2018 Apr 11.