Department of Biotechnology, Hankyong National University, 327 Jungang-ro Anseong-si, Gyeonggi-do 17579, Republic of Korea.
AceEMzyme Co. Ltd., Academic Industry Cooperation, 327 Jungang-ro Anseong-si17579, Gyeonggi-do, 17579, Republic of Korea.
Int J Syst Evol Microbiol. 2020 Apr;70(4):2211-2216. doi: 10.1099/ijsem.0.003950. Epub 2020 Mar 2.
An aerobic and Gram-stain-negative bacterial strain, designated UKS-15, was isolated from lake water in the Republic of Korea. Results of 16S rRNA gene sequence and phylogenetic analyses indicated that the novel isolate belongs to the genus and was most closely related to RCML-52 (98.0 %), 9 NM-14 (97.4 %) and FJY8 (97.2 %). The DNA G+C content was 69.1 mol%. Strain UKS-15 possessed ubiquinone-8 (Q-8) as the sole respiratory quinone and the fatty acid profile comprised iso-C, iso-C and summed feature 9 (iso-C ω9 and/or C 10-methyl) as its major components. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and one unidentified aminophospholipid. Moreover, the physiological and biochemical results and low level of DNA-DNA relatedness (<22.0 %) allowed the phenotypic and genotypic differentiation of strain UKS-15 from other species. Therefore, on the basis of the data from this polyphasic taxonomic study, strain UKS-15 should represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is UKS-15 (=JCM 30983=KACC 18719).
一株需氧、革兰氏阴性细菌菌株，命名为 UKS-15，从韩国的湖水分离得到。16S rRNA 基因序列和系统发育分析的结果表明，该新分离株属于 属，与 RCML-52（98.0%）、 9NM-14（97.4%）和 FJY8（97.2%）最为密切相关。DNA G+C 含量为 69.1mol%。菌株 UKS-15 含有泛醌-8（Q-8）作为唯一的呼吸醌，脂肪酸谱由 iso-C、iso-C 和特征 9（iso-C ω9 和/或 C 10-甲基）组成。主要极性脂为磷脂酰乙醇胺、磷脂酰甘油、双磷脂酰甘油和一种未鉴定的氨基磷脂。此外，生理生化结果和低水平的 DNA-DNA 同源性（<22.0%）允许菌株 UKS-15 与其他 种之间的表型和基因型分化。因此，基于多相分类研究的数据，菌株 UKS-15 应代表 属的一个新种，提议采用 sp. nov.作为其名称。模式菌株为 UKS-15（=JCM 30983=KACC 18719）。
