School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
Department of Bio and Environmental Technology, Seoul Women's University, Seoul, 01797, Republic of Korea.
Arch Microbiol. 2020 Apr;202(3):637-643. doi: 10.1007/s00203-019-01776-z. Epub 2019 Nov 27.
A bacterial strain isolated from a soil collected in Jeju Island, designated as 17J7-1, was Gram-negative, rod-shaped, yellow colored, and motile by gliding. This strain was able to grow at temperature range from 10 to 42 °C, pH 7-9, and tolerated up to 1% NaCl. Analysis of 16S rRNA sequence identified strain 17J7-1 as a member of the genus Lysobacter with close sequence similarity with Lysobacter mobilis 9NM-14 (97.4%), Lysobacter xinjiangensis RCML-52 (97.0%), and Lysobacter humi FJY8 (96.9%). The genomic DNA G + C content of the isolate was 67.9 mol%. DNA-DNA relatedness between strain 17J7-1 and L. mobilis, L. humi, and L. xinjiangensis were 42.3%, 39.5%, and 35.8%, respectively, clearly showing that the isolate is distinct from its closest phylogenetic neighbors in the genus Lysobacter. Average nucleotide identity (ANI) and digital DNA-DNAhybridization (dDDH) values between strain 17J7-1 and L. enzymogenes ATCC 29487, the type species of this genus, and several other close Lysobacter species were less than 77% and 22%, respectively. Major fatty acids were C iso (29.8%), summed feature 9 (C iso ω9c/C 10-methyl; 20.1%), and C iso (17.7%). The predominant respiratory quinone was ubiquinone Q-8 and the major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. In the light of the polyphasic evidence accumulated in this study, strain 17J7-1 is considered to represent a novel species in the genus Lysobacter, for which name Lysobacter terrigena sp. nov. is proposed. The type strain is 17J7-1 (= KCTC 62217 = JCM 33057).
从济州岛采集的土壤中分离到一株细菌，命名为 17J7-1，为革兰氏阴性、杆状、黄色、可滑行运动。该菌株能在 10-42°C、pH7-9 的温度范围内生长，并能耐受高达 1%的 NaCl。16S rRNA 序列分析表明，菌株 17J7-1 是 Lysobacter 属的一个成员，与 Lysobacter mobilis 9NM-14（97.4%）、Lysobacter xinjiangensis RCML-52（97.0%）和 Lysobacter humi FJY8（96.9%）的序列相似性较高。该分离株的基因组 DNA G+C 含量为 67.9mol%。17J7-1 与 L. mobilis、L. humi 和 L. xinjiangensis 之间的 DNA-DNA 同源性分别为 42.3%、39.5%和 35.8%，表明该分离株与 Lysobacter 属中最接近的系统发育邻居明显不同。17J7-1 与 L. enzymogenes ATCC 29487（该属的模式种）和其他几个密切相关的 Lysobacter 种之间的平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）值分别小于 77%和 22%。主要脂肪酸为 C iso（29.8%）、总和特征 9（C iso ω9c/C 10-甲基；20.1%）和 C iso（17.7%）。主要呼吸醌为 ubiquinone Q-8，主要极性脂为磷脂酰乙醇胺、磷脂酰甘油和双磷脂酰甘油。根据本研究积累的多相证据，菌株 17J7-1 被认为代表 Lysobacter 属的一个新种，建议将其命名为 Lysobacter terrigena sp. nov.。模式菌株为 17J7-1（=KCTC 62217=JCM 33057）。
