Department of Life Science, Chung-Ang University, Seoul, 06974, Republic of Korea.
J Microbiol. 2021 Aug;59(8):709-717. doi: 10.1007/s12275-021-1156-y. Epub 2021 Jun 1.
Two Gram-stain negative, yellow-pigmented, and mesophilic bacteria, designated strains R7 and R19, were isolated from sandy and forest soil, South Korea, respectively. Both strains were non-motile rods showing catalase- and oxidase-positive activities. Both strains were shown to grow at 10-37°C and pH 6.0-9.0, and in the presence of 0-1.5% (w/v) NaCl. Strain R7 contained iso-C, iso-C, iso-C, and summed feature 9 (comprising C 10-methyl and/or iso-Cω9c), whereas strain R19 contained iso-C 3-OH, Cω7c alcohol, iso-C, iso-C, iso-C, and summed feature 9 (comprising C 10-methyl and/or iso-Cω9c) as major cellular fatty acids (> 5%). Both strains contained ubiquin-one-8 as the sole isoprenoid quinone and phosphatidylglycerol, phosphatidylethanolamine, and an unidentified phospholipid as the major polar lipids. The DNA G + C contents of strains R7 and R19 calculated from their genomes were 66.9 mol% and 68.9 mol%, respectively. Strains R7 and R19 were most closely related to Lysobacter panacisoli C8-1 and Lysobacter niabensis GH34-4 with 98.7% and 97.8% 16S rRNA sequence similarities, respectively. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains R7 and R19 formed distinct phylogenetic lineages within the genus Lysobacter. Based on phenotypic, chemotaxonomic, and molecular features, strains R7 and R19 represent novel species of the genus Lysobacter, for which the names Lysobacter arenosi sp. nov. and Lysobacter solisilvae sp. nov. are proposed. The type strains of L. arenosi and L. solisilvae are R7 (= KACC 21663 = JCM 34257) and R19 (= KACC 21767 = JCM 34258), respectively.
两株革兰氏阴性、黄色着色、中温细菌，分别命名为 R7 株和 R19 株，分别从韩国的沙土和森林土壤中分离得到。两株菌均为无动力杆状，过氧化氢酶和氧化酶均呈阳性。两株菌均能在 10-37°C、pH6.0-9.0 和 0-1.5%（w/v）NaCl 条件下生长。R7 株含有 iso-C15:0、iso-C16:0、iso-C17:0 和总和特征 9（包括 C10-甲基和/或 iso-Cω9c），而 R19 株含有 iso-C13:0-OH、Cω7c 醇、iso-C15:0、iso-C16:0、iso-C17:0 和总和特征 9（包括 C10-甲基和/或 iso-Cω9c）作为主要的细胞脂肪酸（>5%）。两株菌均含有醌-8 作为唯一的异戊二烯醌，磷脂酰甘油、磷脂酰乙醇胺和一种未鉴定的磷脂作为主要的极性脂。根据基因组计算，R7 株和 R19 株的 DNA G+C 含量分别为 66.9 mol%和 68.9 mol%。R7 株和 R19 株与 Lysobacter panacisoli C8-1 和 Lysobacter niabensis GH34-4 的 16S rRNA 序列相似性最高，分别为 98.7%和 97.8%。基于 16S rRNA 基因序列的系统发育分析表明，R7 株和 R19 株在 Lysobacter 属内形成了独特的系统发育分支。基于表型、化学分类和分子特征，R7 株和 R19 株代表 Lysobacter 属的新种，分别命名为 Lysobacter arenosi sp. nov.和 Lysobacter solisilvae sp. nov.。L. arenosi 和 L. solisilvae 的模式菌株分别为 R7（=KACC 21663=JCM 34257）和 R19（=KACC 21767=JCM 34258）。
