Lee So-Yeon, Kim Pil Soo, Sung Hojun, Hyun Dong-Wook, Bae Jin-Woo
Department of Biology and Department of Biomedical and Pharmaceutical Sciences, Kyung Hee University, Seoul, 02447, Republic of Korea.
Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul, 02447, Republic of Korea.
J Microbiol. 2022 May;60(5):469-477. doi: 10.1007/s12275-022-1647-5. Epub 2022 Mar 31.
Two Gram-stain-negative, mesophilic, strictly aerobic, nonspore forming, and yellow-pigmented strains with rod-shaped cells, designated H21R20 and H23M41, were isolated from the faeces of an Oriental stork (Ciconia boyciana). Based on 16S rRNA gene sequences, both strains showed the highest similarity (98.3-98.4%) to the type strain of Lysobacter concretionis. Phylogenetic analysis based on the 16S rRNA genes and 92 bacterial core genes showed that strains H21R20 and H23M41 were robustly clustered with L. concretionis Ko07. Whole genome sequencing revealed that the genomes of both strains were approximately 2.9 Mb in size. The DNA G + C contents of the H21R20 and H23M41 strains were 67.3 and 66.6%, respectively. The two strains showed 80.1-81.7% average nucleotide identity with L. concretionis Ko07. Strain H21R20 grew optimally at 30°C and pH 8.0 and in the presence of 0.5-3% (wt/vol) NaCl, while strain H23M41 grew optimally at 30°C and pH 7.0-8.0 and in the presence of 0-3% (wt/vol) NaCl. Both strains possessed iso-C, iso-C and summed feature 9 (iso-Cω9c and/or C10-methyl) as the major cellular fatty acids, ubiquinone Q-8 as a predominant quinone, and diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine as the major polar lipids. A multifaceted investigation demonstrated that strains H21R20 and H23M41 represent novel species of the genus Lysobacter, for which we propose the names Lysobacter ciconiae sp. nov. and Lysobacter avium sp. nov. for strains H21R20 (= KCTC 82316 = JCM 34832) and H23M41 (= KCTC 62676 = JCM 33223), respectively.
从一只东方白鹳（Ciconia boyciana）的粪便中分离出两株革兰氏阴性、嗜温、严格需氧、不形成芽孢且带有黄色色素的菌株，细胞呈杆状，分别命名为H21R20和H23M41。基于16S rRNA基因序列，两株菌株与粘细菌（Lysobacter concretionis）模式菌株的相似度最高（98.3 - 98.4%）。基于16S rRNA基因和92个细菌核心基因的系统发育分析表明，H21R20和H23M41菌株与粘细菌Ko07紧密聚类。全基因组测序显示，两株菌株的基因组大小约为2.9 Mb。H21R20和H23M41菌株的DNA G + C含量分别为67.3%和66.6%。这两株菌株与粘细菌Ko07的平均核苷酸同一性为80.1 - 81.7%。H21R20菌株在30°C、pH 8.0以及0.5 - 3%（重量/体积）NaCl存在的条件下生长最佳，而H23M41菌株在30°C、pH 7.0 - 8.0以及0 - 3%（重量/体积）NaCl存在的条件下生长最佳。两株菌株均以异-C、异-C和总特征9（异-Cω9c和/或C10-甲基）作为主要细胞脂肪酸，泛醌Q-8作为主要醌类，双磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺作为主要极性脂质。多方面研究表明，H21R20和H23M41菌株代表了粘细菌属的新物种，我们分别为H21R20（= KCTC 82316 = JCM 34832）和H23M41（= KCTC 62676 = JCM 33223）菌株提议命名为粘鹳菌（Lysobacter ciconiae sp. nov.）和粘鸟菌（Lysobacter avium sp. nov.）。
