1 Department of Food Technology, Korea University , Seoul, Republic of Korea.
J Med Food. 2013 Nov;16(11):977-83. doi: 10.1089/jmf.2012.2739. Epub 2013 Sep 28.
In this study, the protective effect of sweet potato extract against hydrogen peroxide-induced oxidative stress and cytotoxicity on the pheochromocytoma cell line (PC12) was investigated. The active component of the sweet potato extract was purified and determined to be 2,4-di-tert-butylphenol. The antioxidant capacity of 2,4-di-tert-butylphenol was measured by using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical. To examine the effects of 2,4-di-tert-butylphenol on amyloid-beta peptide (Aβ1₁₋₄₂)-induced learning and memory impairment in mice, in vivo behavioral tests were performed. Administration of 2,4-di-tert-butylphenol increased alternation behavior in mice injected with Aβ₁₋₄₂. These results suggest that sweet potato extract could be protective against Aβ-induced neurotoxicity, possibly due to the antioxidative capacity of its constituent, 2,4-di-tert-butylphenol.
本研究旨在探讨甘薯提取物对过氧化氢诱导的氧化应激和细胞毒性对嗜铬细胞瘤细胞系（PC12）的保护作用。甘薯提取物的有效成分被分离并鉴定为 2,4-二叔丁基苯酚。2,2'-连氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基法测定 2,4-二叔丁基苯酚的抗氧化能力。为了研究 2,4-二叔丁基苯酚对 Aβ1₁₋₄₂诱导的小鼠学习和记忆障碍的影响，进行了体内行为测试。给予 2,4-二叔丁基苯酚可增加注射 Aβ₁₋₄₂的小鼠的交替行为。这些结果表明，甘薯提取物可能对 Aβ 诱导的神经毒性具有保护作用，这可能与其成分 2,4-二叔丁基苯酚的抗氧化能力有关。
