Zhang Meng-Zhao, Han Hai-Hao, Zhang Shao-Ze, Wang Cheng-Yun, Lu Yun-Xiang, Zhu Wei-Hong
Key Laboratory for Advanced Materials and Institute of Fine Chemicals, School of Chenmistry and Malecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, P. R. China.
J Mater Chem B. 2017 Nov 28;5(44):8780-8785. doi: 10.1039/c7tb02323e. Epub 2017 Oct 31.
In this work, a new reversible colorimetric and fluorescent probe for sequential recognition of copper ions and biothiols is synthesized easily. Based on the chelation-enhanced fluorescence quenching (CHEQ) effect, this probe shows high sensitivity and selectivity towards Cu, which can be detected by the naked eye. And this experimental phenomenon can also be realised upon addition of biothiols, restoring their initial fluorescence intensity. This probe also features a very high response speed (less than 5 seconds) and a large stokes shift (178 nm) toward Cu and biothiols. Moreover, the detection limit for Cu and biothiols is as low as 7.34 nM and 10.3 nM, respectively. Additionally, this ON-OFF-ON-type fluorescence recognition cycle can be repeated more than 5 times by addition of Cu and biothiols in turn. Particularly, this 1-Cu ensemble is further successfully applied for GSH detection in living cells.
在这项工作中，一种用于顺序识别铜离子和生物硫醇的新型可逆比色和荧光探针很容易合成。基于螯合增强荧光猝灭（CHEQ）效应，该探针对铜具有高灵敏度和选择性，可用肉眼检测。加入生物硫醇后也会出现这种实验现象，恢复其初始荧光强度。该探针还具有非常高的响应速度（小于5秒）以及对铜和生物硫醇的大斯托克斯位移（178 nm）。此外，对铜和生物硫醇的检测限分别低至7.34 nM和10.3 nM。此外，通过依次加入铜和生物硫醇，这种开-关-开型荧光识别循环可以重复5次以上。特别地，这种1-Cu组合进一步成功应用于活细胞中谷胱甘肽（GSH）的检测。
