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[物种名称1]和[物种名称2]的完整叶绿体基因组:基因组结构、比较与系统发育分析

Complete Chloroplast Genomes of and : Genome Structures, Comparative and Phylogenetic Analysis.

作者信息

Munyao Jacinta N, Dong Xiang, Yang Jia-Xin, Mbandi Elijah M, Wanga Vincent O, Oulo Millicent A, Saina Josphat K, Musili Paul M, Hu Guang-Wan

机构信息

CAS key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China.

Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 430074, China.

出版信息

Plants (Basel). 2020 Mar 1;9(3):296. doi: 10.3390/plants9030296.

Abstract

The genus includes many economically important species well-known for medicinal, ornamental, and horticultural values. However, to date, few molecular genomic resources have been reported for this genus. Therefore, there is limited knowledge of phylogenetic studies, and the available chloroplast (cp) genome of () does not provide enough information on this genus. In this study, we present genomic resources for and , which had lengths of 154,248 and 154,154 base pairs (bp), respectively. They had a pair of inverted repeats (IRa and IRb) of 26,114 and 26,254 bp each in size, separating the large single-copy (LSC) region of 84,004 and 83,686 bp from the small single-copy (SSC) region of 18,016 and 17,960 bp in and , respectively. There were 112 distinct genes in each cp genome, which were comprised of 78 protein-coding genes, 30 tRNA genes, and four rRNA genes. The comparative analysis with five other selected species displayed a generally high level of sequence resemblance in structural organization, gene content, and arrangement. Additionally, the phylogenetic analysis confirmed the previous phylogeny and produced a phylogenetic tree with similar topology. It showed that the species (. , . and ) were clustered together in the same clade with a closer relationship than other plants to the . This research, therefore, presents valuable records for further molecular evolutionary and phylogenetic studies which help to fill the gap in genomic resources and resolve the taxonomic complexes of the genus.

摘要

该属包含许多具有重要经济价值的物种,以其药用、观赏和园艺价值而闻名。然而,迄今为止,关于该属的分子基因组资源报道较少。因此,系统发育研究的知识有限,且已有的(某物种)叶绿体(cp)基因组并未提供关于该属足够的信息。在本研究中,我们展示了(两个物种)的基因组资源,其长度分别为154,248和154,154碱基对(bp)。它们各自有一对大小为26,114和26,254 bp的反向重复序列(IRa和IRb),分别将(两个物种)中大小为84,004和83,686 bp的大单拷贝(LSC)区域与大小为18,016和17,960 bp的小单拷贝(SSC)区域分开。每个cp基因组中有112个不同的基因,由78个蛋白质编码基因、30个tRNA基因和4个rRNA基因组成。与其他五个选定物种的比较分析显示,在结构组织、基因内容和排列方面,序列相似性总体较高。此外,系统发育分析证实了先前的系统发育关系,并产生了拓扑结构相似的系统发育树。结果表明,(该属的某些物种)(具体物种名称)聚集在同一个分支中,与其他植物相比,它们与(另一个物种)的关系更密切。因此,本研究为进一步的分子进化和系统发育研究提供了有价值的记录,有助于填补基因组资源的空白,并解决该属的分类学复杂性问题。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/343b/7154914/ec4e72ce9c31/plants-09-00296-g001.jpg

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