Department of Obstetrics and Gynecology, In Vitro Fertilization Unit, Hacettepe University Faculty of Medicine, 2th Floor, 06230, Ankara, Turkey.
Department of Histology and Embryology, Gazi University Medical Faculty. Gazi University Faculty of Medicine Dean's Building, 4th Floor, Beşevler, Yenimahalle, 06560, Ankara, Turkey.
Environ Sci Pollut Res Int. 2020 May;27(14):16231-16245. doi: 10.1007/s11356-020-08041-7. Epub 2020 Mar 2.
BPA, one of the environmental endocrine disruptors, and fructose, reason of liver steatosis which is frequently encountered in the daily diet, contribute to the formation of metabolic syndrome (MetS). This study examines the possible effects of concurrent fructose and BPA administration on MetS and determines the effects of melatonin on this process. In the seven identified groups, a total of forty-two adult male Sprague Dawley rats were treated by following fructose, BPA, and melatonin amounts, separately and together: group 1 (control), group 2 (10% aqueous fructose), group 3 (25 mg/kg BPA), group 4 (10% fructose + 25 mg/kg BPA), group 5 (10% fructose + 20 mg/kg melatonin), group 6 (25 mg/kg BPA + 20 mg/kg melatonin), and group 7 (10% fructose + 25 mg/kg BPA + 20 mg/kg melatonin). At the end of 60 days, histochemical, immunohistochemical, and biochemical procedures were performed on liver tissue. As a result, it was seen that BPA and fructose + BPA induced morphological alteration and inflammation and increased intracellular lipid quantity and amount of collagen and reticular fibers. The percentage of apoptotic liver cells stained by annexin V-FITC/PI was lower in group 7 compared to the group 4 (p < 0,001) and also in group 6 compared to the group 3 (p = 0.014). Both BPA and fructose application caused an increase in lipid peroxidation level due to the increase of oxidative stress. Application of melatonin induced antioxidant enzyme activity and reduced lipid peroxidation level. Our results indicate that fructose and BPA administration triggered the formation of MetS, whereas melatonin healed these variations, although not entirely.
双酚 A（BPA）是环境内分泌干扰物之一，而果糖是日常生活中常见的脂肪肝病因，两者共同导致了代谢综合征（MetS）的形成。本研究旨在探讨同时给予果糖和 BPA 对 MetS 的可能影响，并确定褪黑素对这一过程的影响。在这 7 个确定的组别中，总共 42 只成年雄性 Sprague Dawley 大鼠分别接受了以下果糖、BPA 和褪黑素剂量的处理：第 1 组（对照组）、第 2 组（10% 水溶液果糖）、第 3 组（25mg/kg BPA）、第 4 组（10% 果糖+25mg/kg BPA）、第 5 组（10% 果糖+20mg/kg 褪黑素）、第 6 组（25mg/kg BPA+20mg/kg 褪黑素）和第 7 组（10% 果糖+25mg/kg BPA+20mg/kg 褪黑素）。60 天后，对肝脏组织进行了组织化学、免疫组织化学和生化程序。结果表明，BPA 和果糖+BPA 导致了形态改变和炎症，并增加了细胞内脂质、胶原和网状纤维的含量。与第 4 组相比，第 7 组经 annexin V-FITC/PI 染色的凋亡肝细胞比例较低（p<0.001），与第 3 组相比，第 6 组也较低（p=0.014）。BPA 和果糖的应用均由于氧化应激的增加而导致脂质过氧化水平升高。褪黑素的应用诱导了抗氧化酶的活性，降低了脂质过氧化水平。我们的结果表明，果糖和 BPA 的应用触发了 MetS 的形成，而褪黑素虽然不能完全治愈这些变化，但能改善这些变化。
