Department of Medical Immunology and Allergy, Student Research Committee, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Graduate Research Trainee in Mater Research Institute-University of Queensland, Translational Research Institute, South Brisbane, Australia
Curr Mol Pharmacol. 2020;13(4):306-317. doi: 10.2174/1874467213666200303130153.
Vitamin D insufficiency and deficiency can be associated with adverse effects on pregnancy outcomes, which may include recurrent pregnancy loss through the mechanisms that are yet unknown. The aim of this study was to evaluate the effect of 1,25VitD3 on regulatory T cells (Tregs) and T helper17 (Th17) cell populations In vitro in unexplained recurrent pregnancy loss (URPL) patients and healthy women.
Samples from 20 non-pregnant women with a history of URPL were compared to 20 normal non-pregnant women. Peripheral blood mononuclear cells (PBMC) were divided into 3 wells for each subject: in the presence of 1, 25 VitD3 (50 nM, for 16 hours), PHA (positive control) (10μM), and without any treatment (as a baseline or negative control). The percentage of regulatory T cells and Th17 cells was measured by flow cytometry at baseline and then after cell culture experiments.
Our study indicated that the percentage of Tregs in patients with URPL was significantly lower than the control group (2.42 ± 0.27 vs. 3.41 ± 0.29, P= 0.01). The percentage of Th17 cells was significantly greater in URPL patients compared to the control group (2.91 ± 0.33 vs. 1.18± 0.15, P=0.001). 1, 25VitD3 treatment significantly increased the percentage of Tregs from the baseline in the URPL group compared to that in the control group (1.23 ± 0.03 vs. 1.00 ± 0.03, P= 0.01).
Vitamin D deficiency may be a contributor to recurrent pregnancy loss and suggests supplementation of women with Vit D pre-pregnancy may be protective against URPL.
维生素 D 不足和缺乏与妊娠结局不良有关，其可能通过未知机制导致复发性妊娠丢失。本研究旨在评估 1,25VitD3 对不明原因复发性妊娠丢失（URPL）患者和健康女性体外调节性 T 细胞（Tregs）和辅助性 T 细胞 17（Th17）细胞群的影响。
将 20 名有 URPL 病史的非孕妇与 20 名正常非孕妇进行比较。将外周血单核细胞（PBMC）分为 3 个孔，每个孔用于一个个体：在 1,25 VitD3（50 nM，16 小时）、PHA（阳性对照）（10μM）和无任何处理（作为基线或阴性对照）的存在下。在基线和细胞培养实验后，通过流式细胞术测量调节性 T 细胞和 Th17 细胞的百分比。
我们的研究表明，URPL 患者的 Tregs 百分比明显低于对照组（2.42 ± 0.27 对 3.41 ± 0.29，P=0.01）。URPL 患者的 Th17 细胞百分比明显高于对照组（2.91 ± 0.33 对 1.18± 0.15，P=0.001）。与对照组相比，URPL 组经 1,25VitD3 治疗后 Tregs 百分比从基线显著增加（1.23 ± 0.03 对 1.00 ± 0.03，P=0.01）。
维生素 D 缺乏可能是复发性妊娠丢失的一个原因，并表明孕妇补充维生素 D 可能对 URPL 具有保护作用。
