Department of Biology, Science and Arts University, Yazd, Iran.
Reproductive Immunology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
JBRA Assist Reprod. 2022 Apr 17;26(2):232-236. doi: 10.5935/1518-0557.20210062.
This current survey investigated the role of the Forkhead 3 box protein (foxp3) gene and serum vitamin D levels in women with recurrent spontaneous abortion (RSA).
The mRNA level of the foxp3 gene in peripheral blood was evaluated in women with a history of RSA (N=40) and in controls (N=40) via quantitative polymerase chain reaction. We employed the enzyme-linked immunosorbent assay to assess the serum levels of 1,25-dihydroxyvitamin D3 (1,25(OH)2 D) in both groups. The Mann-Whitney U test and Pearson's correlation coefficient were used to statistically compare study groups between and within themselves, respectively.
Although mRNA levels of foxp3 were higher in women with RSA than in controls, we observed no significant change in mRNA levels of foxp3 between the two groups (p=0.16). An important positive correlation was observed between foxp3 mRNA levels and 1,25(OH)2 D in controls (p=0.003). In contrast, the correlation between foxp3 expression and 1,25(OH)2 D was not significant in the case group (p=0.14). Serum vitamin D levels were lower in women with RSA than in controls (p<0.001).
Our findings demonstrated that 1,25Vitamin D3 along with other molecules might help prevent RSA by providing for an anti-inflammatory state not necessarily through foxp3 expression or T cell differentiation.
本研究旨在探讨叉头框蛋白 3 盒（foxp3）基因和血清维生素 D 水平在复发性自然流产（RSA）妇女中的作用。
通过定量聚合酶链反应评估 RSA 病史妇女（N=40）和对照组妇女（N=40）外周血中 foxp3 基因的 mRNA 水平。我们采用酶联免疫吸附试验评估两组血清 1,25-二羟维生素 D3（1,25(OH)2 D）水平。Mann-Whitney U 检验和 Pearson 相关系数分别用于比较两组间和组内的统计学差异。
尽管 RSA 妇女的 foxp3 mRNA 水平高于对照组，但两组间 foxp3 mRNA 水平无显著差异（p=0.16）。在对照组中，foxp3 mRNA 水平与 1,25(OH)2 D 之间存在重要的正相关（p=0.003）。相比之下，病例组中 foxp3 表达与 1,25(OH)2 D 之间的相关性不显著（p=0.14）。RSA 妇女的血清维生素 D 水平低于对照组（p<0.001）。
我们的研究结果表明，1,25 维生素 D3 可能与其他分子一起通过提供抗炎状态来预防 RSA，而不一定通过 foxp3 表达或 T 细胞分化。
