Marine College, Shandong University, Weihai, 264209, Shandong, China.
State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, Shandong, China.
Arch Microbiol. 2020 Oct;202(8):2127-2133. doi: 10.1007/s00203-020-01933-9. Epub 2020 Jun 6.
A Gram-stain-negative, strictly aerobic, non-flagellated, gliding, rod-shaped bacterium, designated C1 was isolated from the coastal sediment of Xiaoshi Island, Weihai, China. Phylogenetic analysis of the 16S rRNA gene and the genome sequence of the newly isolated strain revealed that it belonged to the genus Psychroflexus within the family Flavobacteriaceae. The 16S rRNA gene sequence similarities between strain C1 and the type strains of Psychroflexus members ranged from 91.6 to 94.9%. The AAI, POCP, ANI and dDDH values between strain C1 and Psychroflexus torquis ATCC 700755 were 67.0%, 53.1%, 70.5% and 20.2%, respectively. The DNA G+C content was 34.0 mol%. Strain C1 grows optimally at 28-30 ℃, at pH 7.5-8.0 and with 3.0-5.0% (w/v) NaCl, and its colonies were orange-colored, convex and circular on the MA plate. Positive for hydrolysis of Tween 20 and catalase activities. The dominant respiratory quinone was menaquinone-6, and the major fatty acids were iso-C 3-OH and iso-C. The polar lipids of strain C1 consisted of phosphatidylethanolamine and three unidentified lipids. On the basis of phylogenetic, phenotypic and chemotaxonomic data, it is considered that strain C1 represents a novel species within the genus Psychroflexus, for which, the name Psychroflexus maritimus sp. nov., is proposed. The type strain is C1 (= MCCC 1H00415 = KCTC 72796).
一株革兰氏阴性、严格需氧、不具鞭毛、滑行、杆状的细菌，被命名为 C1，是从中国威海小石岛的沿海沉积物中分离得到的。16S rRNA 基因和新分离菌株的基因组序列的系统发育分析表明，它属于黄杆菌科黄杆菌属。菌株 C1 与 Psychroflexus 成员的模式菌株的 16S rRNA 基因序列相似性为 91.6%至 94.9%。菌株 C1 与 Psychroflexus torquis ATCC 700755 的 AAI、POCP、ANI 和 dDDH 值分别为 67.0%、53.1%、70.5%和 20.2%。DNA G+C 含量为 34.0 mol%。菌株 C1 在 28-30°C、pH 7.5-8.0 和 3.0-5.0%（w/v）NaCl 下生长最佳，其菌落为 MA 平板上橙色、凸起和圆形。能够水解吐温 20 和过氧化氢酶活性。主要的呼吸醌是menaquinone-6，主要脂肪酸是 iso-C3-OH 和 iso-C。菌株 C1 的极性脂由磷脂酰乙醇胺和三种未鉴定的脂组成。基于系统发育、表型和化学分类学数据，认为菌株 C1 代表黄杆菌属的一个新种，建议将其命名为 Psychroflexus maritimus sp. nov.。模式菌株为 C1（= MCCC 1H00415 = KCTC 72796）。
