Al-Ghafari Ayat B, Balamash Khadijah S, Al Doghaither Huda A
Department of Biochemistry, Faculty of Science, King Abdulaziz University, P.O.Box 80200, Jeddah 21589, Saudi Arabia.
Experimental Biochemistry Unit, King Fahd Medical Research Center, King Abdulaziz University, P.O. Box 3270, Jeddah, 22252 Saudi Arabia.
Saudi J Biol Sci. 2020 Mar;27(3):827-832. doi: 10.1016/j.sjbs.2020.01.006. Epub 2020 Jan 21.
Colorectal cancer (CRC) is one of the leading causes of mortality and morbidity worldwide, and there has been a significant increase in the incidence of CRC in recent decades. Therefore, there is an urgent need to identify blood biomarkers that can be used for early diagnosis. It is not yet clear whether the level of vitamin D and its receptor, vitamin D receptor (VDR), in the blood are helpful factors in the diagnosis of CRC. Therefore, the study focuses on determining the VDR serum level's contribution and other chemical parameters to the risk of CRC. A total of 189 Saudi participants (66 CRC patients and 123 control patients) aged 20-80 years old were enrolled in this case-control study. A serum sample was collected from each participant, and the levels of VDR and other bone profile tests were determined using ELISA or chemiluminescent assays. values < 0.05 were considered statistically significant. The results showed a highly significant reduction in the levels of total vitamin D ( < 0.0001), VDR ( < 0.0001), vitamin D ( < 0.05), and calcium ( < 0.0001) in the serum of CRC patients compared to the controls. However, the alkaline phosphatase level was higher in CRC patients compared to the controls ( < 0.0001). None of the blood markers showed a significant correlation to the progression of CRC ( > 0.05). More investigation is needed to elucidate different physiological processes that can be affected by these blood biomarkers, therefore changing the carcinogenesis of CRC.
结直肠癌（CRC）是全球死亡率和发病率的主要原因之一，近几十年来CRC的发病率显著增加。因此，迫切需要识别可用于早期诊断的血液生物标志物。血液中维生素D及其受体维生素D受体（VDR）的水平是否有助于CRC的诊断尚不清楚。因此，该研究聚焦于确定VDR血清水平以及其他化学参数对CRC风险的影响。本病例对照研究共纳入了189名年龄在20至80岁之间的沙特参与者（66名CRC患者和123名对照患者）。从每位参与者采集血清样本，使用酶联免疫吸附测定（ELISA）或化学发光测定法测定VDR水平和其他骨代谢指标。P值<0.05被认为具有统计学意义。结果显示，与对照组相比，CRC患者血清中的总维生素D（P<0.0001）、VDR（P<0.0001）、维生素D（P<0.05）和钙（P<0.0001）水平显著降低。然而，与对照组相比，CRC患者的碱性磷酸酶水平更高（P<0.0001）。没有一种血液标志物与CRC的进展存在显著相关性（P>0.05）。需要更多研究来阐明这些血液生物标志物可能影响的不同生理过程，从而改变CRC的致癌过程。
