Activity Anticancer n-hexane Fraction of . Rhizome to Breast Cancer MCF-7 Cell Line.

BACKGROUND

Cancer is one of the causes of morbidity and mortality worldwide. Breast cancer is one of the most common types of cancer in Indonesia. Failures that often occur in the treatment of cancer primarily through chemotherapy, synthetic drugs that have side effects include anemia, alopecia, cardiotoxic and hepatotoxic due to low anti-cancer selectivity and unclear carcinogenesis process. . rhizome is one of the medicinal plants that potential enough to be developed as an anticancer agent.

AIM

The aim of this study was to anticancer activity n-hexane fraction . rhizomes to breast cancer MCF-7 cell line in vitro.

METHODS

. rhizomes powder was extracted ethanol by percolation then fractionated with n-hexane. Phytochemical screening was then carried out. The cytotoxic activity of the n-hexane fraction was determined by observing this extract on MCF-7 cells using the (MTT). Selectivity index (IS) of normal cells (Vero cells). Cell cycle and apoptosis induction were analyzed by flow cytometry.

RESULTS

The result showed that the fraction n-hexane . rhizome has anticancer activity against breast cancer MCF-7 cells with accumulation cell cycle in the G0-G1 phase and through induction of apoptosis.

CONCLUSION

The n-hexane fraction . rhizome has potent anticancer activity.