Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.
Chin Med. 2012 Jun 10;7(1):15. doi: 10.1186/1749-8546-7-15.
Six herbs in the Plant Genetics Conservation Project that have been used as complementary medicines were chosen on the basis of their medicinal value, namely Terminalia mucronata, Diospyros winitii, Bridelia insulana, Artabotrys harmandii, Terminallia triptera, and Croton oblongifolius. This study aims to evaluate the potential anticancer activity of 50% ethanol-water extracts of these six herbs.
Fifty percent ethanol-water crude extracts of the six herbs were prepared. The cytotoxicity of the herbal extracts relative to that of melphalan was evaluated using a hepatoma cell line (HepG2), and examined by neutral red assays and apoptosis induction by gel electrophoresis and flow cytometry after 24 h.
A significant difference was found between the cytotoxicity of the 50% ethanol-water crude extracts and melphalan (P = 0.000). The 50% ethanol-water crude extracts of all six herbs exhibited cytotoxicity against HepG2 cells, with IC50 values ranging from 100 to 500 μg/mL. The extract of T. triptera showed the highest cytotoxicity with an IC50 of 148.7 ± 12.3 μg/mL, while melphalan had an IC50 of 39.79 ± 7.62 μg/mL. The 50% ethanol-water crude extracts of D. winitii and T. triptera, but not A. harmandii, produced a DNA ladder. The 50% ethanol-water crude extracts of D. winitii, T. triptera, and A. harmandii induced apoptosis detected by flow cytometry.
The 50% ethanol-water crude extracts of D. winitii, T. triptera, and A. harmandii showed anticancer activity in vitro.
选择了植物遗传学保护项目中六种具有药用价值的草药作为补充药物，它们分别是榄李、乌榄、布渣叶、鹰不泊、三叉苦和算盘子。本研究旨在评估这六种草药的 50%乙醇-水提取物的潜在抗癌活性。
制备了六种草药的 50%乙醇-水粗提取物。通过中性红测定法，检测肝癌细胞系（HepG2）对草药提取物的细胞毒性，评估其相对氨甲喋呤的细胞毒性，并通过凝胶电泳和流式细胞术在 24 小时后观察细胞凋亡诱导。
50%乙醇-水粗提取物的细胞毒性与氨甲喋呤之间存在显著差异（P=0.000）。六种草药的 50%乙醇-水粗提取物均对 HepG2 细胞表现出细胞毒性，IC50 值范围为 100 至 500μg/ml。鹰不泊提取物的细胞毒性最高，IC50 为 148.7±12.3μg/ml，而氨甲喋呤的 IC50 为 39.79±7.62μg/ml。D. winitii 和 T. triptera 的 50%乙醇-水粗提取物，但不是 A. harmandii，产生了 DNA 梯。通过流式细胞术检测到 D. winitii、T. triptera 和 A. harmandii 的 50%乙醇-水粗提取物诱导了细胞凋亡。
D. winitii、T. triptera 和 A. harmandii 的 50%乙醇-水粗提取物在体外显示出抗癌活性。
