Center for Bio-Healthcare Materials, Bio-Convergence Materials R&D Division, Korea Institute of Ceramic Engineering and Technology, Cheongju, 28160, Republic of Korea.
Department of Applied Bioengineering, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, 08826, Republic of Korea.
Int J Nanomedicine. 2024 Aug 16;19:8403-8415. doi: 10.2147/IJN.S452636. eCollection 2024.
L. (CR) is traditionally used in medicine for its anti-inflammatory properties. In particular, α-cyperone, which is isolated from the essential oil and found primarily in the n-hexane fraction of the ethanolic extract, is known to inhibit NO production in LPS-stimulated RAW 264.7 cells. However, high concentrations of α-cyperone are required for sufficient anti-inflammatory activity. Even, essential oil obtained from has the disadvantage of low solubility and stability in aqueous environment, which makes it difficult to be applied in various fields and easily loses its activity. Therefore, in this study, we aimed to increase the extraction yield of by microbubble extraction and prepare nanoparticles (NPs) that can preserve its activity in a stable and bioavailable manner by utilizing nanoprecipitation.
rhizomes were extracted in 50% ethanol using microbubbles and then fractionated with n-hexane to obtain α-cyperone-rich n-hexane fraction (CRHF). The biodegradable plant extract, α-cyperone, was prepared as green nanoparticles (CR@NPs) by nanoprecipitation technique under mild reaction conditions. The physicochemical properties of CR@NPs, including size, polydispersity index, and surface charge, were determined using dynamic light scattering. The extraction yield and encapsulation efficiency of α-cyperone were quantified by high-performance liquid chromatography. Antioxidant and anti-inflammatory activities were evaluated by DPPH assay and in vitro ROS and NO assays, and biocompatibility was assessed by MTT assay.
loaded nanoparticles demonstrated overcoming the limitation of α-cyperone solubility and stability in CRHF and also the antioxidant, anti-inflammatory properties as evidenced by in vitro assays in cellular models.
The versatility of green chemistry, such as α-cyperone, enables the production of nanoparticles with promising biomedical applications such as cosmetics, pharmaceuticals, and food products.
L.（CR）在医学上传统上因其抗炎特性而使用。特别是，从精油中分离出来的α-环酮，主要存在于乙醇提取物的正己烷部分，已知可抑制 LPS 刺激的 RAW 264.7 细胞中 NO 的产生。然而，需要高浓度的α-环酮才能发挥足够的抗炎活性。即使是从 中获得的精油，也存在在水相环境中溶解度和稳定性低的缺点，这使得它难以在各个领域中应用，并且容易失去其活性。因此，在这项研究中，我们旨在通过微泡提取提高 的提取产率，并通过利用纳米沉淀技术制备能够以稳定和可生物利用的方式保存其活性的纳米粒子（NPs）。
使用微泡从 50%乙醇中提取根茎，然后用正己烷进行分馏，得到富含α-环酮的正己烷部分（CRHF）。可生物降解的植物提取物α-环酮通过纳米沉淀技术在温和的反应条件下制备成绿色纳米粒子（CR@NPs）。使用动态光散射法测定 CR@NPs 的物理化学性质，包括粒径、多分散指数和表面电荷。通过高效液相色谱法定量测定α-环酮的提取产率和包封效率。通过 DPPH 测定法和体外 ROS 和 NO 测定法评估抗氧化和抗炎活性，并通过 MTT 测定法评估生物相容性。
负载的纳米粒子证明克服了 CRHF 中 α-环酮溶解度和稳定性的限制，并且通过细胞模型的体外试验证明了抗氧化、抗炎特性。
绿色化学的多功能性，如α-环酮，能够生产出具有有前途的生物医学应用的纳米粒子，如化妆品、制药和食品产品。
