Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China.
Technical Innovation Center for Exploitation of Marine Biological Resources, Ministry of Natural Resources, Xiamen 361005, China.
Mar Drugs. 2021 Dec 9;19(12):699. doi: 10.3390/md19120699.
In this study, the effects of a polysaccharide derived from (LJP) on obesity were investigated in mice fed a high-fat diet (HFD). LJP significantly attenuated obesity-related features, lowering serum triglycerides, glucose, total cholesterol and low-density lipoprotein cholesterol levels. HFD-induced liver steatosis and hepatocellular ballooning were significantly attenuated by LJP. Additionally, LJP was found to significantly modulate hepatic gene expressions of AMPK and HMGCR, which are key regulators of lipid and cholesterol metabolism. We further found that LJP ameliorated HFD-induced gut microbiota (GM) dysbiosis by significantly reducing the obesity-related Firmicutes to Bacteroidetes ratio, meanwhile promoting the growth of Verrucomicrobia at the phylum level. At the genus level, propionate-producing bacteria and were elevated by LJP, which might explain the result that LJP elevated fecal propionate concentration. Taken together, these findings suggest that dietary intake of LJP modulates hepatic energy homeostasis to alleviate obesity-related nonalcoholic fatty liver disease associated with GM regulation.
在这项研究中，研究了来源于（LJP）的多糖对高脂饮食（HFD）喂养小鼠肥胖的影响。LJP 显著减轻了肥胖相关特征，降低了血清甘油三酯、葡萄糖、总胆固醇和低密度脂蛋白胆固醇水平。LJP 显著减轻了 HFD 诱导的肝脂肪变性和肝细胞气球样变。此外，研究发现 LJP 显著调节了肝脏 AMPK 和 HMGCR 的基因表达，这是脂质和胆固醇代谢的关键调节因子。我们进一步发现，LJP 通过显著降低肥胖相关的厚壁菌门到拟杆菌门的比例，同时促进疣微菌门在门水平上的生长，改善了 HFD 诱导的肠道微生物群（GM）失调。在属水平上，LJP 增加了产生丙酸盐的细菌和，这可能解释了 LJP 提高粪便丙酸盐浓度的结果。总之，这些发现表明，饮食摄入 LJP 可调节肝脏能量稳态，减轻与 GM 调节相关的肥胖相关非酒精性脂肪性肝病。
