Koĭcheva N, Mushmov D
Vutr Boles. 1988;27(4):66-8.
The urines of 33 patients with various renal diseases and proteinuria from 0.5 up to 4.7 g/l were examined by isoelectric focusing in flat polyacrylamide gel with pH range of 3.5-10. According to the type of the individual proteins excreted in the urine, identified by immunoelectrophoresis, the following types of proteinuria were differentiated: physiological--4 patients, moderately selective--26 patients, and feebly selective--3 patients. The isoelectric focusing of the urine revealed that the urine proteins were distributed in pH range 3.5-6.3. The lower the proteinuria, selectivity--the more proteins are found by isofocusing and the more they engage the cathode part of the pH-gradient. 22 urines can be examined simultaneously. The isofocusing allows the differentiation of the proteinuria and yields results well comparable with those of the immunoelectrophoresis.
对33例患有各种肾脏疾病且蛋白尿水平在0.5至4.7g/l之间的患者尿液，采用在pH范围为3.5 - 10的平板聚丙烯酰胺凝胶中进行等电聚焦的方法进行检测。根据免疫电泳鉴定出的尿液中排出的个体蛋白质类型，区分出以下几种蛋白尿类型：生理性蛋白尿——4例患者，中度选择性蛋白尿——26例患者，轻度选择性蛋白尿——3例患者。尿液的等电聚焦显示，尿蛋白分布在pH 3.5 - 6.3范围内。蛋白尿水平越低、选择性越高——通过等聚焦发现的蛋白质就越多，且它们越集中在pH梯度的阴极部分。可同时检测22份尿液。等聚焦能够区分蛋白尿，其结果与免疫电泳的结果具有良好的可比性。
