Department of Plastic Surgery, Peking University Third Hospital, Beijing, China.
Int J Low Extrem Wounds. 2021 Jun;20(2):135-142. doi: 10.1177/1534734620907978. Epub 2020 Mar 4.
The discovery of stromal vascular fraction cells and platelet-rich plasma in promoting tissue regeneration has prompted a new idea for the treatment of chronic diabetic ulcer of the lower limb. The study aim was to evaluate the clinical efficacy of a new method that applied stromal vascular fraction cells and platelet-rich plasma together in the treatment of recalcitrant chronic diabetic ulcer. We conducted a single-center, prospective, open, noncontrolled study. Four patients (5 ulcers in total) who had received standard treatment for diabetic ulcer for at least 3 months that failed to heal was enrolled. All patients were treated with surgical debridement, cell suspension (stromal vascular fraction cells suspended by platelet-rich plasma) injection into the wound, and platelet-rich plasma gel coverage. Wounds were measured every week after treatment using a 2-dimensional digital camera and a 3-dimensional wound measurement device. All patients were followed-up for 4 months after the treatment. Four of the 5 ulcers healed completely within a mean of 71.75 ± 29.57 days. The average proportion of granulation tissue achieved 100% within 4 weeks for all cases. The wound size decreased to less than half of the original size for all cases 4 weeks after the treatment. Findings revealed that the new treatment is efficient to achieve wound healing in patients with recalcitrant chronic diabetic ulcer of lower limb.
基质血管成分细胞和富含血小板的血浆在促进组织再生方面的发现，为下肢慢性糖尿病溃疡的治疗带来了新的思路。本研究旨在评估一种新方法的临床疗效，即将基质血管成分细胞和富含血小板的血浆联合应用于治疗难治性慢性糖尿病溃疡。我们进行了一项单中心、前瞻性、开放性、非对照研究。共纳入 4 名（5 处溃疡）接受标准糖尿病溃疡治疗至少 3 个月但未愈合的患者。所有患者均接受手术清创、细胞悬液（富含血小板的血浆悬浮基质血管成分细胞）注射入创面和富含血小板的血浆凝胶覆盖治疗。治疗后每周使用二维数码相机和三维创面测量仪测量创面。所有患者在治疗后均随访 4 个月。5 处溃疡中有 4 处平均 71.75±29.57 天内完全愈合。所有患者在 4 周内肉芽组织的平均比例达到 100%。治疗 4 周后，所有患者的创面大小均缩小至原来的一半以下。结果表明，该新治疗方法对下肢难治性慢性糖尿病溃疡患者的创面愈合有效。
