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富血小板血浆/阿魏酸水凝胶促进和修复大鼠退变椎间盘。

Enhancement and Repair of Degenerative Intervertebral Disc in Rats Using Platelet-Rich Plasma/Ferulic Acid Hydrogel.

机构信息

The Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot, China.

Department of Region A, Spinal Surgery Center, The Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot, China.

出版信息

Cartilage. 2023 Dec;14(4):506-515. doi: 10.1177/19476035231157341. Epub 2023 Mar 10.

DOI:10.1177/19476035231157341
PMID:36899464
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10807731/
Abstract

BACKGROUND

Intervertebral degenerative disc (IDD) disease is one of the most common clinical conditions causing low back pain. The main objective of this study was to investigate the repair effect of platelet-rich plasma (PRP) and ferulic acid (FA) hydrogel compound on degenerative discs in rats in combination with bioengineering technology, which may provide a strong theoretical basis for the future treatment of IDD.

METHODS

Forty-five male Sprague-Dawley rats were randomly divided into groups A-F; MRI was performed in each group at 0, 4, and 8 weeks after injection; and disc tissues were obtained after executing the animals. The histomorphology, apoptosis, and protein synthesis of intervertebral discs in each group were observed by hematoxylin-eosin, Masson, terminal deoxynucleotidyl transferase dUTP nick end labeling staining, and Western blot.

RESULTS

The release concentration of all groups reached the peak at 12 hours, and the highest concentration was found in the hydrogel/PRP/FA group at the same time. The MTT assay showed that hydrogel/PRP/FA is well-cytocompatible. The results of animal experiments show that hydrogel/PRP/FA has a good effect on degenerative intervertebral disc in rats.

CONCLUSION

PRP/FA-rich hydrogel compound plays an active role in promoting extracellular matrix synthesis, strengthening and repairing degenerated intervertebral discs in rats.

摘要

背景

椎间盘退行性病变（IDD）疾病是引起腰痛的最常见临床病症之一。本研究的主要目的是结合生物工程技术，研究富血小板血浆（PRP）和阿魏酸（FA）水凝胶复合物对大鼠退变椎间盘的修复作用，为未来治疗 IDD 提供强有力的理论依据。

方法

45 只雄性 Sprague-Dawley 大鼠随机分为 A-F 组；每组在注射后 0、4 和 8 周进行 MRI 检查；处死动物后获取椎间盘组织。通过苏木精-伊红、Masson、末端脱氧核苷酸转移酶 dUTP 缺口末端标记染色和 Western blot 观察各组椎间盘的组织形态学、细胞凋亡和蛋白合成。

结果

所有组的释放浓度在 12 小时达到峰值，同时在水凝胶/PRP/FA 组中达到最高浓度。MTT 检测表明水凝胶/PRP/FA 具有良好的细胞相容性。动物实验结果表明，水凝胶/PRP/FA 对大鼠退变椎间盘有较好的疗效。

结论

富含 PRP/FA 的水凝胶复合物在促进细胞外基质合成、加强和修复大鼠退变椎间盘方面发挥着积极作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fed/10807731/e39e8e65d55f/10.1177_19476035231157341-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fed/10807731/f7768a29cebf/10.1177_19476035231157341-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fed/10807731/ec4cb6f73de4/10.1177_19476035231157341-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fed/10807731/089f50391472/10.1177_19476035231157341-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fed/10807731/b2c8fe8cb404/10.1177_19476035231157341-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fed/10807731/9d2db70e6975/10.1177_19476035231157341-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fed/10807731/e39e8e65d55f/10.1177_19476035231157341-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fed/10807731/f7768a29cebf/10.1177_19476035231157341-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fed/10807731/ec4cb6f73de4/10.1177_19476035231157341-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fed/10807731/089f50391472/10.1177_19476035231157341-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fed/10807731/b2c8fe8cb404/10.1177_19476035231157341-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fed/10807731/9d2db70e6975/10.1177_19476035231157341-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fed/10807731/e39e8e65d55f/10.1177_19476035231157341-fig6.jpg

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