Division of Infectious Diseases, Department of Medicine, Weill Cornell Medicine, New York, New York, USA
J Clin Microbiol. 2019 Jan 30;57(2). doi: 10.1128/JCM.01608-18. Print 2019 Feb.
Polymyxins are relied upon for the treatment of carbapenem-resistant Gram-negative bacterial infections, but polymyxin resistance is increasing. Only broth microdilution is recommended for polymyxin susceptibility testing, but this method is impractical for most clinical microbiology laboratories. An article in this issue of the (P. J. Simner, Y. Bergman, M. Trejo, A. A. Roberts, R. Marayan, T. Tekle, S. Campeau, A. Kazmi, D. Bell, S. Lewis, P. D. Tamma, R. Humphries, and J. A. Hindler, J Clin Microbiol 57:e01163-18, 2019, https://doi.org/10.1128/JCM.01163-18) found that colistin broth disk elution, a method that requires only colistin disks and broth, had excellent performance compared to broth microdilution for all strains except -positive strains.
黏菌素用于治疗碳青霉烯类耐药革兰氏阴性菌感染,但黏菌素耐药性正在增加。仅推荐肉汤微量稀释法用于黏菌素药敏试验,但该方法对大多数临床微生物学实验室来说不切实际。本期《临床微生物学杂志》(P. J. Simner、Y. Bergman、M. Trejo、A. A. Roberts、R. Marayan、T. Tekle、S. Campeau、A. Kazmi、D. Bell、S. Lewis、P. D. Tamma、R. Humphries 和 J. A. Hindler,J Clin Microbiol 57:e01163-18, 2019, https://doi.org/10.1128/JCM.01163-18)发现,仅需黏菌素药敏纸片和肉汤的黏菌素肉汤纸片洗脱法,除 -阳性 菌株外,与肉汤微量稀释法相比,对所有菌株均具有出色的性能。