Rezaei Farimani Azam, Goodarzi Mohammad Taghi, Saidijam Massoud, Yadegarazari Reza, Zarei Sadegh, Asadi Soheila
Noncommunicable Diseases Research Center, Neyshabur University of Medical Sciences, Neyshabur, Iran.
Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Iran J Basic Med Sci. 2019 Dec;22(12):1408-1414. doi: 10.22038/IJBMS.2019.13988.
Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex proteins are involved in membrane trafficking. The expression of isoforms of SNAP-23, syntaxin-4, and VAMP-2 is significantly done in skeletal muscles; they control GLUT4 trafficking. It is believed that type 2 diabetes could be caused by the modifications in the expression of SNARE complex proteins. The purpose of this study was to evaluate the effect of resveratrol on the expression of these proteins in type 2 diabetes.
Forty male Wistar rats were selected. Streptozotocin and nicotinamide were applied for the induction of type 2 diabetes. The animals were divided into five groups. Healthy and diabetic groups were set as control; resveratrol (1, 5, and 10 mg/kg body weight) was applied to treat the three groups of diabetic rats for 30 days. Real-time qRT-PCR was applied to evaluate the expression of SNARE complex proteins.
There is a link between diabetes and insulin resistance and up-regulation of SNARE proteins expression. Resveratrol improved hyperglycemia and insulin resistance along with a non-significant reduction in the expression of SNARE proteins.
Increased expression of SNARE proteins was possibly a compensatory mechanism in response to insulin resistance in the skeletal muscles of diabetic rats. Resveratrol non-significantly reduced the expression of SNARE proteins by enhancing insulin sensitivity, where this effect was dose-dependent. Thus, higher doses of resveratrol and longer intervention periods could probably be more effective. Another molecular mechanism of the anti-diabetic properties of resveratrol was identified with an effect on the expression of SNARE proteins.
可溶性N - 乙基马来酰亚胺敏感因子附着蛋白受体（SNARE）复合体蛋白参与膜运输。SNAP - 23、 syntaxin - 4和VAMP - 2的异构体在骨骼肌中表达显著；它们控制葡萄糖转运蛋白4（GLUT4）的运输。据信，2型糖尿病可能由SNARE复合体蛋白表达的改变引起。本研究的目的是评估白藜芦醇对2型糖尿病中这些蛋白表达的影响。
选取40只雄性Wistar大鼠。应用链脲佐菌素和烟酰胺诱导2型糖尿病。将动物分为五组。健康组和糖尿病组设为对照组；白藜芦醇（1、5和10mg/kg体重）用于治疗三组糖尿病大鼠30天。应用实时定量逆转录聚合酶链反应（qRT - PCR）评估SNARE复合体蛋白的表达。
糖尿病与胰岛素抵抗以及SNARE蛋白表达上调之间存在关联。白藜芦醇改善了高血糖和胰岛素抵抗，同时SNARE蛋白表达有非显著性降低。
SNARE蛋白表达增加可能是糖尿病大鼠骨骼肌对胰岛素抵抗的一种代偿机制。白藜芦醇通过增强胰岛素敏感性非显著性降低了SNARE蛋白的表达，且这种作用呈剂量依赖性。因此，更高剂量的白藜芦醇和更长的干预时间可能会更有效。确定了白藜芦醇抗糖尿病特性的另一种分子机制，即对SNARE蛋白表达的影响。
