Ahmadvand Hassan, Mahdavifard Sina
Razi Herbal Researches Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
Int J Prev Med. 2019 Oct 9;10:176. doi: 10.4103/ijpvm.IJPVM_396_17. eCollection 2019.
We investigated the effect of thioctic acid (TA) on kidney function, oxidative stress, and inflammatory status in serum and kidney homogenates of a rat subjected to ischemia-reperfusion injury (IRI).
Thirty male Wistar rats were randomly divided into three equal groups: sham, IR, and IR + TA in 50 mg/kg once-daily intraperitoneal injection for 2 weeks, before IR induction. The levels of urea and creatinine (Cr) in the serum of rats were measured. Malondialdehyde and nitric oxide (NO) as stress oxidative markers; tumor necrosis factor-α, interleukin-6, and myeloperoxidase as inflammatory markers, as well as activities of superoxide dismutase, glutathione peroxidase and catalase, and glutathione (GSH) level in both serum and kidney homogenates were determined.
Cr and urea increased in serum of IR group. Furthermore, levels of oxidative stress and inflammatory markers in serum and kidney homogenates of the cited group were higher than the sham group. TA not only decreased the levels of Cr, urea, oxidative stress, and inflammation but also elevated the level of GSH and activities of antioxidant enzymes ( < 0.001).
The findings showed that TA protected IR rat against kidney dysfunction and IRI due to reinforcing endogenous antioxidant and subtracting of inflammatory markers.
我们研究了硫辛酸（TA）对遭受缺血再灌注损伤（IRI）的大鼠血清和肾脏匀浆中肾功能、氧化应激及炎症状态的影响。
30只雄性Wistar大鼠随机分为三组，每组10只：假手术组、IRI组、IR + TA组。IR + TA组在诱导IRI前，每天腹腔注射50 mg/kg TA，持续2周。检测大鼠血清中尿素和肌酐（Cr）水平。测定丙二醛和一氧化氮（NO）作为氧化应激标志物；肿瘤坏死因子-α、白细胞介素-6和髓过氧化物酶作为炎症标志物，以及血清和肾脏匀浆中超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶的活性及谷胱甘肽（GSH）水平。
IRI组大鼠血清中Cr和尿素升高。此外，该组大鼠血清和肾脏匀浆中的氧化应激和炎症标志物水平高于假手术组。TA不仅降低了Cr、尿素、氧化应激和炎症水平，还提高了GSH水平及抗氧化酶活性（P < 0.001）。
研究结果表明，TA通过增强内源性抗氧化作用和减少炎症标志物，保护IRI大鼠免受肾功能障碍和IRI的影响。
