School of Dentistry Universidad Nacional de Córdoba Haya de la Torre s/n, Córdoba, Argentina
Med Oral Patol Oral Cir Bucal. 2020 May 1;25(3):e425-e430. doi: 10.4317/medoral.23462.
The Human Papillomavirus (HPV) has different strategies for persist in the cells. This characteristic has led us to consider the presence of the virus in tissues of the oral cavity that had no clinical signs of infection. The aim of this study was to detect the presence of DNA-HPV at multiple sites of the oral cavity.
A case-control study was designed: Oral Squamous Carcinoma Group (OSCG), healthy n=72 and Control Group (CG), n=72, healthy volunteers paired by sex and age with OSCG. Four samples were taken from OSCG: saliva, biopsy, brush scraping of lesion and contralateral healthy side. In CG a saliva sample and a scratch of the posterior border of tongue were collected. HPV was detected by PCR using Bioneer Accuprep genomic DNA Extraction kit, and consensus primers MY09 and MY11. Chi square test was applied.
432 samples were obtained from 144 individuals. DNA-HPV was detected in 30 (42%) of OSCG subjects and 3(4%) of CG. Two or more positive samples were obtained in 67% of the OSCG, 67% in saliva and 60% in biopsy; in CG 100% of the individuals were positive in the two samples.
HPV is frequently present in oral cavity as a multifocal infection, even without the presence of clinical lesions.
人乳头瘤病毒（HPV）有多种在细胞中持续存在的策略。这种特性使我们考虑到病毒在口腔组织中的存在，这些组织没有感染的临床迹象。本研究旨在检测口腔多个部位 HPV 的 DNA 存在情况。
设计了一项病例对照研究：口腔鳞状细胞癌组（OSCG），健康者 n=72 例；对照组（CG），健康志愿者 n=72 例，与 OSCG 按性别和年龄配对。从 OSCG 中采集了四个样本：唾液、活检、病变处的刷擦和对侧健康侧。在 CG 中采集了唾液样本和舌后缘的划痕。使用 Bioneer Accuprep 基因组 DNA 提取试剂盒和通用引物 MY09 和 MY11 通过 PCR 检测 HPV。应用卡方检验。
从 144 个人中获得了 432 个样本。OSCG 中有 30 例（42%）和 CG 中有 3 例（4%）检测到 DNA-HPV。OSCG 中有 67%的个体有两个或更多的阳性样本，唾液中有 67%，活检中有 60%；CG 中 100%的个体在两个样本中均为阳性。
HPV 经常以多灶性感染的形式存在于口腔中，即使没有临床病变存在。
