Biotechnology Process Engineering Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), Cheongju, Korea.
Bio-Evaluation Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), Cheongju, Korea.
FEBS Open Bio. 2020 May;10(5):780-788. doi: 10.1002/2211-5463.12829. Epub 2020 Mar 31.
Volatile compounds can be produced by fermentation from genetically engineered microorganisms. Escherichia coli strains are mainly used for isoprene production owing to their higher titers; however, this has thus far been confined to only strains BL21, BL21 (DE3), Rosetta, and BW25113. Here, we tested four groups of E. coli strains for improved isoprene production, including K-12 (DH5α, BW25113, W3110, MG1655, XL1-Blue, and JM109), B [Rosetta (DE3), BL21, and BL21 (DE3)], Crooks C, and Waksman W strains. The isoprene productivity of BL21 and MG1655 was remarkably higher than that of the others in 5-L fermentation, and scale-up fermentation (300 L) of BL21 was successfully performed. This system shows potential for biobased production of fuel and volatile compounds in industrial applications.
发酵可从基因工程微生物中产生挥发性化合物。由于其较高的效价，大肠杆菌菌株主要用于异戊二烯的生产；然而，到目前为止，这仅局限于菌株 BL21、BL21（DE3）、Rosetta 和 BW25113。在这里，我们测试了四组大肠杆菌菌株以提高异戊二烯的产量，包括 K-12（DH5α、BW25113、W3110、MG1655、XL1-Blue 和 JM109）、B [Rosetta（DE3）、BL21 和 BL21（DE3）]、Crooks C 和 Waksman W 菌株。在 5-L 发酵中，BL21 和 MG1655 的异戊二烯生产力明显高于其他菌株，并且成功地进行了 BL21 的放大发酵（300 L）。该系统显示了在工业应用中生物基生产燃料和挥发性化合物的潜力。
