College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, China.
School of Medicine, Yale University, New Haven, CT, 06511, USA.
Fish Shellfish Immunol. 2020 May;100:238-245. doi: 10.1016/j.fsi.2020.02.071. Epub 2020 Mar 3.
Licorice is a widely used herbal medicine for the treatment of various diseases in southern Europe and parts of Asia. It has been reported that the isoliquiritin (ISL) from Glycyrrhiza root has the activity of promoting angiogenesis. The purpose of this study was to investigate the effect of ISL on the wound healing activity of zebrafish and its mechanism. 6-month-old zebrafish were injured in the skin (2 mm in diameter) and then treated with ISL. By measuring wound size and by histological examination, we found that ISL improved wound healing. In addition, 4-day-old zebrafish embryos of double transgenic line [Tg(fli-1:EGFP)]/[Tg(mpeg:mCherry)] were suffered from tissue traumas and then treated with ISL. Through fluorescent microscopy, we found that ISL promoted macrophage recruitment and angiogenesis in the wound area. Through qPCR analysis, we found that ISL up-regulated the expression of genes related to inflammation and angiogenesis in zebrafish embryos. These results showed that ISL could promote inflammatory response and angiogenesis, which played key roles in promoting wound healing. Therefore, ISL can be used as a promising candidate to promote wound healing.
甘草是一种在欧洲南部和亚洲部分地区广泛用于治疗各种疾病的草药。据报道，甘草根中的异甘草素（ISL）具有促进血管生成的活性。本研究旨在探讨 ISL 对斑马鱼伤口愈合活性的影响及其机制。将 6 月龄的斑马鱼皮肤（直径 2mm）受伤，然后用 ISL 处理。通过测量伤口大小和组织学检查，发现 ISL 可促进伤口愈合。此外，对双转基因系[Tg(fli-1:EGFP)]/[Tg(mpeg:mCherry)]的 4 天大的斑马鱼胚胎进行组织损伤，并给予 ISL 处理。通过荧光显微镜观察，发现 ISL 促进了伤口区域的巨噬细胞募集和血管生成。通过 qPCR 分析，发现 ISL 上调了斑马鱼胚胎中与炎症和血管生成相关的基因表达。这些结果表明，ISL 可以促进炎症反应和血管生成，这在促进伤口愈合中起着关键作用。因此，ISL 可以作为一种有前途的候选药物，促进伤口愈合。
