Kobayashi S, Miyamoto T, Kimura I, Kimura M
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
Biol Pharm Bull. 1995 Oct;18(10):1382-6. doi: 10.1248/bpb.18.1382.
A water extract of licorice root inhibits granuloma angiogenesis in adjuvant-induced chronic inflammation (Phytother. Res., 5, 195. 1991). The present study has investigated the effects of licorice-derived compounds on granuloma angiogenesis. Isoliquiritin (0.31-3.1 mg/kg), a licorice-derived flavonoid, inhibited the carmine content of granuloma tissue 50-fold greater than licorice extract. Glyeyrrhizin (20-80 mg/kg), a licorice-derived saponin, inhibited carmine content with a weak potency. The licorice extract (0.01-1 mg/ml) also inhibited tube formation from vascular endothelial cells in a concentration-dependent manner. From the chemical structure-activities of used licorice-derived flavonoids (0.1-100 microM), their potencies for anti-tube formation were in the order isoliquiritigenin > isoliquiritin > liquiritigenin >> isoliquiritin-apioside. Glycyrrhizin (0.1-100 microM) and glycyrrhetinic acid (0.1-10 microM) increased tube formation. A glycyrrhizin (82 micrograms/ml)-induced increase in tube formation was inhibited by isoliquiritin. The combined effect of a mixture of 82 micrograms/ml glycyrrhizin and 4.2 micrograms/ml isoliquiritin, a similar concentration ratio to their yield ratio in the licorice extract, corresponded to the effect of 100 micrograms/ml extract. In conclusion, the anti-angiogenic effect of licorice extract depended on the anti-tube formation effect of isoliquiritin.
甘草根水提取物可抑制佐剂诱导的慢性炎症中的肉芽肿血管生成（《植物疗法研究》，第5卷，195页，1991年）。本研究调查了甘草衍生化合物对肉芽肿血管生成的影响。异甘草素（0.31 - 3.1毫克/千克），一种甘草衍生的黄酮类化合物，抑制肉芽肿组织中胭脂红含量的能力比甘草提取物强50倍。甘草皂苷（20 - 80毫克/千克），一种甘草衍生的皂苷，抑制胭脂红含量的能力较弱。甘草提取物（0.01 - 1毫克/毫升）也以浓度依赖的方式抑制血管内皮细胞形成管腔。从所使用的甘草衍生黄酮类化合物（0.1 - 100微摩尔）的化学结构 - 活性来看，它们抗管腔形成的效力顺序为异甘草素 > 异甘草苷 > 甘草素 >> 异甘草苷芹菜糖苷。甘草皂苷（0.1 - 100微摩尔）和甘草次酸（0.1 - 10微摩尔）增加管腔形成。异甘草素可抑制甘草皂苷（82微克/毫升）诱导的管腔形成增加。82微克/毫升甘草皂苷和4.2微克/毫升异甘草素的混合物的联合作用，其浓度比与它们在甘草提取物中的产率比相似，相当于100微克/毫升提取物的作用。总之，甘草提取物的抗血管生成作用取决于异甘草素的抗管腔形成作用。
